Molecular characterization of spinach germplasm and a marker linked to downy mildew resistance using AFLPs

AFLPs have been used to characterize genetic diversity in 332 spinach genotypes in the USDA spinach germplasm collection. A total of 64 AFLP primer combinations were screened for their ability to identify polymorphisms among six spinach genotypes initially selected from diverse genetic pedigrees. Th...

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Bibliographic Details
Published in:Phytopathology 2004-06, Vol.94 (6)
Main Authors: Irish, B M, Correll, J C, de los Reyes, B, Feng, C
Format: Article
Language:English
Subjects:
Peronospora farinosa
Spinacia oleracea
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Summary:AFLPs have been used to characterize genetic diversity in 332 spinach genotypes in the USDA spinach germplasm collection. A total of 64 AFLP primer combinations were screened for their ability to identify polymorphisms among six spinach genotypes initially selected from diverse genetic pedigrees. The AFLP procedure yielded 16-124 reliably scorable fragments per primer combination. Several primer combinations, which yielded a high number of polymorphic fragments, were selected to evaluate the accessions in the germplams collection. The AFLP technique was a robust method for characterizing genetic diversity of spinach. The AFLP procedure was also used to identify polymorphisms among progeny of a cross between a downy mildew (Peronospora farinosa f. sp. spinaciae) susceptible and resistant parent (BC3). A single AFLP marker was found to be linked to the gene(s) controlling resistance to race 5 and 6 of the pathogen.
ISSN:0031-949X