UvsX Recombinase and Dda Helicase Rescue Stalled Bacteriophage T4 DNA Replication Forks in Vitro

The rescue of stalled replication forks via a series of steps that include fork regression, template switching, and fork restoration often has been proposed as a major mechanism for accurately bypassing non-coding DNA lesions. Bacteriophage T4 encodes almost all of the proteins required for its own...

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Bibliographic Details
Published in:The Journal of biological chemistry 2004-08, Vol.279 (34), p.35735-35740
Main Authors: Kadyrov, Farid A, Drake, John W
Format: Article
Language:English
Subjects:
Bacteriophage T4 - genetics
Bacteriophage T4 - metabolism
Base Sequence
DNA Helicases - genetics
DNA Helicases - metabolism
DNA Replication
DNA, Viral - biosynthesis
DNA, Viral - genetics
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Membrane Proteins - genetics
Membrane Proteins - metabolism
Molecular Sequence Data
Phage T4
Recombination, Genetic
Viral Proteins - genetics
Viral Proteins - metabolism
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Summary:The rescue of stalled replication forks via a series of steps that include fork regression, template switching, and fork restoration often has been proposed as a major mechanism for accurately bypassing non-coding DNA lesions. Bacteriophage T4 encodes almost all of the proteins required for its own DNA replication, recombination, and repair. Both recombination and recombination repair in T4 rely on UvsX, a RecA-like recombinase. We show here that UvsX plus the T4-encoded helicase Dda suffice to rescue stalled T4 replication forks in vitro . This rescue is based on two sequential template-switching reactions that allow DNA replication to bypass a non-coding DNA lesion in a non-mutagenic manner.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M403942200