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UvsX Recombinase and Dda Helicase Rescue Stalled Bacteriophage T4 DNA Replication Forks in Vitro
The rescue of stalled replication forks via a series of steps that include fork regression, template switching, and fork restoration often has been proposed as a major mechanism for accurately bypassing non-coding DNA lesions. Bacteriophage T4 encodes almost all of the proteins required for its own...
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| Published in: | The Journal of biological chemistry 2004-08, Vol.279 (34), p.35735-35740 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c436t-7545e2122ea6b5ed88deb495e16ea10eb8ca68c04e4601b5ee5063e6ad1f39f33 |
| container_end_page | 35740 |
| container_issue | 34 |
| container_start_page | 35735 |
| container_title | The Journal of biological chemistry |
| container_volume | 279 |
| creator | Kadyrov, Farid A Drake, John W |
| description | The rescue of stalled replication forks via a series of steps that include fork regression, template switching, and fork restoration
often has been proposed as a major mechanism for accurately bypassing non-coding DNA lesions. Bacteriophage T4 encodes almost
all of the proteins required for its own DNA replication, recombination, and repair. Both recombination and recombination
repair in T4 rely on UvsX, a RecA-like recombinase. We show here that UvsX plus the T4-encoded helicase Dda suffice to rescue
stalled T4 replication forks in vitro . This rescue is based on two sequential template-switching reactions that allow DNA replication to bypass a non-coding DNA
lesion in a non-mutagenic manner. |
| doi_str_mv | 10.1074/jbc.M403942200 |
| format | article |
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often has been proposed as a major mechanism for accurately bypassing non-coding DNA lesions. Bacteriophage T4 encodes almost
all of the proteins required for its own DNA replication, recombination, and repair. Both recombination and recombination
repair in T4 rely on UvsX, a RecA-like recombinase. We show here that UvsX plus the T4-encoded helicase Dda suffice to rescue
stalled T4 replication forks in vitro . This rescue is based on two sequential template-switching reactions that allow DNA replication to bypass a non-coding DNA
lesion in a non-mutagenic manner.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M403942200</identifier><identifier>PMID: 15194689</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Bacteriophage T4 - genetics ; Bacteriophage T4 - metabolism ; Base Sequence ; DNA Helicases - genetics ; DNA Helicases - metabolism ; DNA Replication ; DNA, Viral - biosynthesis ; DNA, Viral - genetics ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Molecular Sequence Data ; Phage T4 ; Recombination, Genetic ; Viral Proteins - genetics ; Viral Proteins - metabolism</subject><ispartof>The Journal of biological chemistry, 2004-08, Vol.279 (34), p.35735-35740</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-7545e2122ea6b5ed88deb495e16ea10eb8ca68c04e4601b5ee5063e6ad1f39f33</citedby><cites>FETCH-LOGICAL-c436t-7545e2122ea6b5ed88deb495e16ea10eb8ca68c04e4601b5ee5063e6ad1f39f33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15194689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kadyrov, Farid A</creatorcontrib><creatorcontrib>Drake, John W</creatorcontrib><title>UvsX Recombinase and Dda Helicase Rescue Stalled Bacteriophage T4 DNA Replication Forks in Vitro</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The rescue of stalled replication forks via a series of steps that include fork regression, template switching, and fork restoration
often has been proposed as a major mechanism for accurately bypassing non-coding DNA lesions. Bacteriophage T4 encodes almost
all of the proteins required for its own DNA replication, recombination, and repair. Both recombination and recombination
repair in T4 rely on UvsX, a RecA-like recombinase. We show here that UvsX plus the T4-encoded helicase Dda suffice to rescue
stalled T4 replication forks in vitro . This rescue is based on two sequential template-switching reactions that allow DNA replication to bypass a non-coding DNA
lesion in a non-mutagenic manner.</description><subject>Bacteriophage T4 - genetics</subject><subject>Bacteriophage T4 - metabolism</subject><subject>Base Sequence</subject><subject>DNA Helicases - genetics</subject><subject>DNA Helicases - metabolism</subject><subject>DNA Replication</subject><subject>DNA, Viral - biosynthesis</subject><subject>DNA, Viral - genetics</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Phage T4</subject><subject>Recombination, Genetic</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpF0ElPwzAQBWALgWhZrhyRD4hbivfGRyirxCJBi3ozjjOlhiQudgri35OqlZjLSKNv3uEhdETJgJKhOPso3OBBEK4FY4RsoT4lOc-4pNNt1CeE0UwzmffQXkofpBuh6S7qUUm1ULnuo7fJd5riZ3ChLnxjE2DblPiytPgWKu9Wh2dIbgn4pbVVBSW-sK6F6MNibt8BjwW-fDzvzGKlWx8afB3iZ8K-wa--jeEA7cxsleBws_fR5PpqPLrN7p9u7kbn95kTXLXZUAoJjDIGVhUSyjwvoRBaAlVgKYEid1bljggQitBOgCSKg7IlnXE943wfna5zFzF8LSG1pvbJQVXZBsIyGZoToZhmHRysoYshpQgzs4i-tvHXUGJWnZquU_PfafdwvEleFjWU_3xTYgdO1mDu3-c_PoIpfHBzqA0basOF4XLIJf8D4O99gQ</recordid><startdate>20040820</startdate><enddate>20040820</enddate><creator>Kadyrov, Farid A</creator><creator>Drake, John W</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20040820</creationdate><title>UvsX Recombinase and Dda Helicase Rescue Stalled Bacteriophage T4 DNA Replication Forks in Vitro</title><author>Kadyrov, Farid A ; Drake, John W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-7545e2122ea6b5ed88deb495e16ea10eb8ca68c04e4601b5ee5063e6ad1f39f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Bacteriophage T4 - genetics</topic><topic>Bacteriophage T4 - metabolism</topic><topic>Base Sequence</topic><topic>DNA Helicases - genetics</topic><topic>DNA Helicases - metabolism</topic><topic>DNA Replication</topic><topic>DNA, Viral - biosynthesis</topic><topic>DNA, Viral - genetics</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Phage T4</topic><topic>Recombination, Genetic</topic><topic>Viral Proteins - genetics</topic><topic>Viral Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kadyrov, Farid A</creatorcontrib><creatorcontrib>Drake, John W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kadyrov, Farid A</au><au>Drake, John W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>UvsX Recombinase and Dda Helicase Rescue Stalled Bacteriophage T4 DNA Replication Forks in Vitro</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2004-08-20</date><risdate>2004</risdate><volume>279</volume><issue>34</issue><spage>35735</spage><epage>35740</epage><pages>35735-35740</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The rescue of stalled replication forks via a series of steps that include fork regression, template switching, and fork restoration
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all of the proteins required for its own DNA replication, recombination, and repair. Both recombination and recombination
repair in T4 rely on UvsX, a RecA-like recombinase. We show here that UvsX plus the T4-encoded helicase Dda suffice to rescue
stalled T4 replication forks in vitro . This rescue is based on two sequential template-switching reactions that allow DNA replication to bypass a non-coding DNA
lesion in a non-mutagenic manner.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>15194689</pmid><doi>10.1074/jbc.M403942200</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 2004-08, Vol.279 (34), p.35735-35740 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | ScienceDirect (Online service) |
| subjects | Bacteriophage T4 - genetics Bacteriophage T4 - metabolism Base Sequence DNA Helicases - genetics DNA Helicases - metabolism DNA Replication DNA, Viral - biosynthesis DNA, Viral - genetics DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Membrane Proteins - genetics Membrane Proteins - metabolism Molecular Sequence Data Phage T4 Recombination, Genetic Viral Proteins - genetics Viral Proteins - metabolism |
| title | UvsX Recombinase and Dda Helicase Rescue Stalled Bacteriophage T4 DNA Replication Forks in Vitro |
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