Isolation and Characterization of Pseudomonas Strain for Degradation of 4-nitrophenol

A Pseudomonas strain capable of growing on 4-nitrophenol (4NP) as a sole source of carbon was isolated from a pharmaceutical waste dumpsite after selective enrichment. Experiments revealed that the strain, SF1, utilizes 4-nitrophenol after 6h of adaptation and could degrade up to 2.1 mM of 4NP. Oxyg...

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Bibliographic Details
Published in:Microbes and Environments 2001, Vol.16(1), pp.49-52
Main Authors: Qureshi, Asifa, Prabu, S. K., Purohit, Hemant J.
Format: Article
Language:English
Subjects:
4-nitrophenol
Key words: biodegradation
Pseudomonas putida
Pseudomonas strain
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Summary:A Pseudomonas strain capable of growing on 4-nitrophenol (4NP) as a sole source of carbon was isolated from a pharmaceutical waste dumpsite after selective enrichment. Experiments revealed that the strain, SF1, utilizes 4-nitrophenol after 6h of adaptation and could degrade up to 2.1 mM of 4NP. Oxygen consumption studies demonstrated that strain SF1 utilized 1,4-benzoquinone and hydroquinone, as well as 4NP. On partial 16S-rDNA sequence analysis, strain SF1 showed highest homology with the Pseudomonas putida strain based on the FASTA program.
ISSN:1342-6311
1347-4405
DOI:10.1264/jsme2.2001.49