Trinucleotide microsatellite loci for the peppered moth (Biston betularia)

Fourteen polymorphic trinucleotide microsatellites were isolated in the peppered moth, Biston betularia, using an enrichment protocol. Moderate to high allelic diversity (three to 22 alleles per locus) was found in a sample from northern England. We are currently using these markers to study the pop...

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Bibliographic Details
Published in:Molecular ecology notes 2004-06, Vol.4 (2), p.179-181
Main Authors: Daly, D, Waltham, K, Mulley, J, Watts, P.C, Rosin, A, Kemp, S.J, Saccheri, I.J
Format: Article
Language:English
Subjects:
alleles
alleles per locus
Biston betularia
DNA primers
genetic markers
Geometridae
heterozygosity
Lepidoptera
loci
microsatellite
microsatellite repeats
molecular sequence data
nucleotide sequences
polymerase chain reaction
polymorphic loci
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Summary:Fourteen polymorphic trinucleotide microsatellites were isolated in the peppered moth, Biston betularia, using an enrichment protocol. Moderate to high allelic diversity (three to 22 alleles per locus) was found in a sample from northern England. We are currently using these markers to study the population structure of B. betularia in northern England, where a cline for melanism still exists.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2004.00607.x