Development of hematin conjugated PLGA nanoparticle for selective cancer targeting

Targeted nanomedicine for cancer therapy has gained widespread popularity and is being extensively explored. Porphyrins have intrinsic tumor localizing ability and have been studied for photodynamic therapy. However, they have not been used as cancer targeting agents for nanomedicines. In this study...

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Bibliographic Details
Published in:European journal of pharmaceutical sciences 2016-08, Vol.91, p.138-143
Main Authors: Amin, Md. Lutful, Kim, Dami, Kim, SeJin
Format: Article
Language:English
Subjects:
Arginine - chemistry
Drug delivery
Drug Delivery Systems
HeLa Cells
Hematin
Hemin - administration & dosage
Hemin - chemistry
Humans
Lactic Acid - administration & dosage
Lactic Acid - chemistry
Nanoparticles - administration & dosage
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Neoplasms - drug therapy
PLGA nanoparticle
Polyglycolic Acid - administration & dosage
Polyglycolic Acid - chemistry
Proton-Coupled Folate Transporter - metabolism
Surface modification
Surface Properties
Targeted nanoparticle
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Summary:Targeted nanomedicine for cancer therapy has gained widespread popularity and is being extensively explored. Porphyrins have intrinsic tumor localizing ability and have been studied for photodynamic therapy. However, they have not been used as cancer targeting agents for nanomedicines. In this study, PLGA nanoparticles were formulated and an iron-containing blood porphyrin, hematin was conjugated to the surface of the nanoparticles to investigate selectivity towards cancer cell and cellular internalization. Hematin was previously shown to facilitate growth and proliferation of cancer cells. PLGA nanoparticles were characterized by FE-SEM, AFM, DLS, and Zeta potential analyzer. The conjugation of hematin was confirmed by FTIR. HeLa cells were used to study tumor selectivity and uptake. Hematin conjugated particles (ζ potential: −15.19mV) showed higher affinity towards the cancer cells than the control particles. The result indicated that the particles were internalized by heme carrier protein-1. Together these data suggest that hematin is a promising cancer targeting material for nanotherapeutics. [Display omitted]
ISSN:0928-0987
1879-0720
DOI:10.1016/j.ejps.2016.05.029