Modulation of zymosan- and phorbol ester-stimulated respiratory burst of rock bream ( Oplegnathus fasciatus) phagocytes by staurosporine, genistein and sodium orthovanadate

The ability of different phosphorylation modulators to modify the opsonized zymosan (OZ)- and phorbol myristate acetate (PMA)-stimulated respiratory burst activity of rock bream ( Oplegnathus fasciatus) head-kidney phagocytes was investigated. OZ-stimulated respiratory burst was significantly inhibi...

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Bibliographic Details
Published in:Aquaculture 2004-08, Vol.237 (1), p.1-7
Main Authors: Lee, Sun Joung, Kim, Sung Mi, Kim, Sung Koo, Nam, Yoon Kwon, Kim, Dong Soo, Kim, Ki Hong
Format: Article
Language:English
Subjects:
Animal aquaculture
Animal productions
Aquaculture
Biological and medical sciences
bream
enzyme inhibition
Fish
Fundamental and applied biological sciences. Psychology
General aspects
genistein
marine fish
Oplegnathus fasciatus
Perciformes
phagocytes
phorbol ester
Phosphorylation
PMA
protein phosphorylation
protein-tyrosine kinases
protein-tyrosine-phosphatase
Respiratory burst
Respiratory system
Rock bream
sodium orthovanadate
staurosporine
Zymosan
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Summary:The ability of different phosphorylation modulators to modify the opsonized zymosan (OZ)- and phorbol myristate acetate (PMA)-stimulated respiratory burst activity of rock bream ( Oplegnathus fasciatus) head-kidney phagocytes was investigated. OZ-stimulated respiratory burst was significantly inhibited by 5 nM of staurosporine, a protein kinase C (PKC) inhibitor, and completely inhibited by 10 nM of staurosporine, whereas PMA-stimulated respiratory burst was completely inhibited by both 5 and 10 nM of staurosporine. Although genistein, a protein tyrosine kinase (PTK) inhibitor, inhibited significantly both OZ- and PMA-stimulated respiratory burst, OZ-stimulated respiratory burst was inhibited much more selectively than PMA-stimulated one. OZ- and PMA-stimulated respiratory burst were increased significantly by sodium orthovanadate, a protein tyrosine phosphatase inhibitor, and the increased rate of OZ-stimulated respiratory burst was markedly higher than that of PMA-stimulated one. These results suggest that OZ-stimulated respiratory burst is more dependent on PTK while the PMA-stimulated one is more dependent on PKC, and the balance between tyrosine kinase and phosphotyrosine phosphatase activity is important in determining the intensity of respiratory burst in phagocytes.
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2004.03.009