Pyrene Metabolism by New Species Isolated from Soil Rhizoctonia Zeae SOL3

• Published in: Water, air, and soil pollution air, and soil pollution, 2015-06, Vol.226 (6), p.1-9, Article 186
• Main Authors: Khudhair, Ameer Badr, Hadibarata, Tony, Yusoff, Abdull Rahim Mohd, Teh, Zee Chuang, Adnan, Liyana Amalina, Kamyab, Hesam
• Format: Article
• Language: English
• Subjects: Acids; Aromatic hydrocarbons; Atmospheric Protection/Air Quality Control/Air Pollution; Bacteria; Benzoic acid; Biodegradation; Carbon; Chromatography; Climate Change/Climate Change Impacts; Discovery and exploration; Earth and Environmental Science; Environment; Environmental monitoring; Enzymes; Experiments; Fungi; Gas chromatography; Glucose; Hydrogeology; Mass spectrometry; Mass spectroscopy; Metabolism; Metabolites; Microorganisms; New species; Organic acids; p-Hydroxybenzoic acid; Polycyclic aromatic hydrocarbons; Pyrene; Rhizoctonia; Sodium chloride; Soil contamination; Soil microorganisms; Soil pollution; Soil Science & Conservation; Thin layer chromatography; Water Quality/Water Pollution
• ISSN: 0049-6979; 1573-2932
• DOI: 10.1007/s11270-015-2432-4

Rhizoctonia zeae SOL3 fungus was isolated from contaminated soil based on its ability to decolorize remazol brilliant blue R in solid medium. This fungus has been used to degrade pyrene a four-ring polycyclic aromatic hydrocarbon. R. zeae SOL3 could biodegrade pyrene as a sole source of carbon and energy. Different parameters were investigated to study their effect on the biodegradation rate. The highest biodegradation rate reached at 28 °C, non-agitated culture, 20 g/L glucose, 24 g/L NaCl, and 20 mg/L pyrene. The metabolites of pyrene were detected by thin layer chromatography (TLC) and confirmed by gas chromatography–mass spectrometry (GC-MS), which were identified as benzoic acid, 4-hydroxybenzoic acid and botanic acid.