CKAP2 is necessary to ensure the faithful spindle bipolarity in a dividing diploid hepatocyte

Spindle bipolarity is crucial for segregating chromosome during somatic cell division. Previous studies have suggested that cytoskeleton associated protein 2 (CKAP2) is involved in spindle assembly and chromosome segregation. In this study, we show that CKAP2-depleted primary hepatocytes exhibit ove...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2016-05, Vol.473 (4), p.886-893
Main Authors: Yoo, Bum Ho, Park, Chi-Hu, Kim, Hyun-Jun, Kang, Du-Seock, Bae, Chang-Dae
Format: Article
Language:English
Subjects:
Animals
Cell Division - genetics
Cells, Cultured
Centrosome
Centrosome - physiology
Chromosome segregation
Chromosome Segregation - genetics
CKAP2
Cytoskeletal Proteins - metabolism
Diploidy
Hepatocytes - cytology
M Phase Cell Cycle Checkpoints - genetics
Male
Mice
Mice, Inbred C57BL
Multipolar cell division
Spindle Apparatus - genetics
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Summary:Spindle bipolarity is crucial for segregating chromosome during somatic cell division. Previous studies have suggested that cytoskeleton associated protein 2 (CKAP2) is involved in spindle assembly and chromosome segregation. In this study, we show that CKAP2-depleted primary hepatocytes exhibit over-duplicated centrosomes with disjoined chromosomes from metaphase plate. These cells proceed to apoptosis or multipolar cell division and subsequent apoptotic cell death. In addition, a mouse liver regeneration experiment showed a marked decrease in efficiency of hepatic regeneration in CKAP2-depleted liver. These data suggest a physiological role of CKAP2 in the formation of spindle bipolarity, which is necessary for maintaining chromosomal stability. •Knockdown of CKAP2 causes over-duplicated centrosomes with disjoined chromosomes from metaphase plate in dividing hepatocytes.•The over-duplicated centrosomes are due to the premature centriole disengagement at the G2/M phase.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2016.03.145