Titanium With Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway

• Published in: Journal of cellular biochemistry 2016-07, Vol.117 (7), p.1718-1726
• Main Authors: M.S. Castro-Raucci, Larissa, S. Francischini, Marcelo, N. Teixeira, Lucas, P. Ferraz, Emanuela, B. Lopes, Helena, T. de Oliveira, Paulo, Hassan, Mohammad Q., Rosa, Adalberto L., Beloti, Marcio M.
• Format: Article
• Language: English
• Subjects: Alkaline Phosphatase - biosynthesis; Animals; BMP; BONE; Bone Morphogenetic Protein 2 - biosynthesis; Bone Morphogenetic Protein 4 - biosynthesis; Bone Morphogenetic Protein Receptors, Type I - metabolism; Cell Differentiation - drug effects; Cell Line; Core Binding Factor Alpha 1 Subunit - metabolism; Gene Expression Regulation - drug effects; Mice; NANOTOPOGRAPHY; OSTEOBLAST; Osteoblasts - metabolism; Signal Transduction - drug effects; Surface Properties; TITANIUM; Titanium - pharmacology
• ISSN: 0730-2312; 1097-4644
• DOI: 10.1002/jcb.25469

ABSTRACT We aimed at evaluating the effect of titanium (Ti) with nanotopography (Nano) on the endogenous expression of BMP‐2 and BMP‐4 and the relevance of this process to the nanotopography‐induced osteoblast differentiation. MC3T3‐E1 cells were grown on Nano and machined (Machined) Ti surfaces and the endogenous BMP‐2/4 expression and the effect of BMP receptor BMPR1A silencing in both osteoblast differentiation and expression of genes related to TGF‐β/BMP signaling were evaluated. Nano supported higher BMP‐2 gene and protein expression and upregulated the osteoblast differentiation compared with Machined Ti surface. The BMPR1A silencing inhibited the osteogenic potential induced by Nano Ti surface as indicated by reduced alkaline phosphatase (ALP), osteocalcin and RUNX2 gene expression, RUNX2 protein expression and ALP activity. In addition, the expression of genes related to TGF‐β/BMP signaling was deeply affected by BMPR1A‐silenced cells grown on Nano Ti surface. In conclusion, we have demonstrated for the first time that nanotopography induces osteoblast differentiation, at least in part, by upregulating the endogenous production of BMP‐2 and modulating BMP signaling pathway. J. Cell. Biochem. 117: 1718–1726, 2016. © 2015 Wiley Periodicals, Inc. Nanotopography of titanium (Ti) surface generated by a simple and low‐cost treatment with H2SO4/H2O2 induces in vitro osteogenesis. This study aimed at evaluating the effect of Ti with nanotopography on the endogenous expression of BMPs and the relevance of this process to the nanotopography‐induced osteoblast differentiation. We have demonstrated for the first time that this nanotopography induces osteoblast differentiation, at least in part, by upregulating the endogenous production of BMP‐2 and modulating BMP signaling pathway.