Validation and designation of quantitative trait loci for seed protein, seed oil, and seed weight from two soybean populations

• Published in: Crop science 2004-07, Vol.44 (4), p.1218-1225
• Main Authors: Fasoula, V.A, Harris, D.K, Boerma, H.R
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Crop science; crossing; Deoxyribonucleic acid; DNA; Factor analysis; Fundamental and applied biological sciences. Psychology; Gene loci; Gene mapping; genetic markers; Genetics and breeding of economic plants; Glycine max; lines; lipid content; microsatellite repeats; Oil; Oils & fats; protein content; Proteins; quantitative trait loci; restriction fragment length polymorphism; seed oils; Seeds; Soybean; Soybeans; Variance analysis; Varietal selection. Specialized plant breeding, plant breeding aims; Weight; Yield, quality, earliness, varia
• ISSN: 0011-183X; 1435-0653
• DOI: 10.2135/cropsci2004.1218

In soybean [Glycine max (L.) Merr.], there is limited and inconsistent information on the confirmation of previously reported QTL. The objectives of this study were to: (i) confirm previously reported QTL for seed protein, seed oil, and seed weight in an independent population of PI97100 x 'Coker 237' with the same RFLP markers and (ii) verify previously reported QTL in an independent population of 'Young' x PI416937 for the same seed traits using SSR markers mapped in the same region as the original RFLP markers. Each population consisted of 176 F(2:4) lines and was grown in randomized complete block trials in two or three different environments. Single-factor analysis of variance was used to verify the QTL that had significant (P less than or equal to 0.01) associations. In the PI97100 x Coker 237 population, two (cqProt-001 and cqProt-002) of four previously described QTL for seed protein, two (cqOil-001 and cqOil-002) of three QTL for oil content, and none of three QTL for seed weight were confirmed in the independent population. In the Young x PI416937 population, none of the three previously reported QTL for protein was confirmed. One (cqOil-003) of three QTL for oil content and two (cqSd wt-001 and cqSd wt-002) of three QTL for seed weight were verified. The unconfirmed QTL may have been false positive or they may have been specific for the sample of lines used in the original populations. These results confirm the necessity of validating QTL in parallel populations before utilizing them in a plant improvement program.