The importance of selecting the right internal control gene to study the effects of antenatal glucocorticoid administration in human placenta

Abstract RT-qPCR requires a suitable set of internal control genes (ICGs) for an accurate normalization. The usefulness of 7 previously published ICGs in the human placenta was analyzed according to the effects of betamethasone treatment, sex and fetal age. Raw RT-qPCR data of the ICGs were evaluate...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2016-08, Vol.44, p.19-22
Main Authors: Gütling, H, Bionaz, M, Sloboda, D.M, Ehrlich, L, Braun, F, Gramzow, A.K, Henrich, W, Plagemann, A, Braun, T
Format: Article
Language:English
Subjects:
B2M
Betamethasone
Co-regulation
Electron Transport Complex II - genetics
Female
Gene Expression Profiling
Genes, Essential - genetics
geNorm
Glucocorticoids
Glucocorticoids - pharmacology
Glucocorticoids - therapeutic use
HMBS
HPRT1
Human placenta
Humans
Hydroxymethylbilane Synthase - genetics
Internal control gene
Internal Medicine
Obstetrics and Gynecology
Peptidylprolyl Isomerase - genetics
Placenta - drug effects
Placenta - metabolism
PPIA
Pregnancy
Receptors, Glucocorticoid - genetics
Ribosomal Proteins - genetics
RPL19
SDHA
YWHAZ
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Summary:Abstract RT-qPCR requires a suitable set of internal control genes (ICGs) for an accurate normalization. The usefulness of 7 previously published ICGs in the human placenta was analyzed according to the effects of betamethasone treatment, sex and fetal age. Raw RT-qPCR data of the ICGs were evaluated using published algorithms. The algorithms revealed that a reliable normalization was achieved using the geometrical mean of PPIA , RPL19 , HMBS and SDHA . The use of a different subset ICGs out of the 7 investigated, although not statistically affected by the conditions, biased the results, as demonstrated through changes in expression of glucocorticoid receptor (NR3C1) mRNA as a target gene.
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2016.05.011