Inactivation of cryptosporidium parvum oocysts using medium- and low-pressure ultraviolet radiation

• Published in: Water research (Oxford) 2001-04, Vol.35 (6), p.1387-1398
• Main Authors: Craik, Stephen A, Weldon, Daniela, Finch, Gordon R, Bolton, James R, Belosevic, Miodrag
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Applied sciences; Cryptosporidium parvum; Cryptosporidium parvum - growth & development; Cryptosporidium parvum - pathogenicity; Cryptosporidium parvum - radiation effects; Dose-Response Relationship, Radiation; Drinking water and swimming-pool water. Desalination; Exact sciences and technology; Mice; Pollution; Temperature; Ultraviolet Rays; ultraviolet, medium-pressure, low-pressure, microorganism reduction, cryptosporidium parvum, infectivity, irradiance; Water - parasitology; Water treatment and pollution
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/S0043-1354(00)00399-7

The effect of ultraviolet radiation from low- and medium-pressure mercury arc lamps on Cryptosporidium parvum oocysts was studied using a collimated beam apparatus. Experiments were conducted using parasites suspended in both filtered surface water and phosphate buffered laboratory water. Inactivation of oocysts was measured as reduction in infectivity using a CD-1 neonatal mouse model and was found to be a non-linear function of UV dose over the range of germicidal doses tested (0.8–119 mJ/cm 2). Oocyst inactivation increased rapidly with UV dose at doses less than 25 mJ/cm 2 with two and three log-units inactivation at approximately 10 and 25 mJ/cm 2, respectively. The cause of significant leveling-off and tailing in the UV inactivation curve at higher doses was not determined. Maximum measured oocyst inactivation ranged from 3.4 to greater than 4.9 log-units and was dependent on different batches of parasites. Water type and temperature, the concentration of oocysts in the suspension, and the UV irradiance did not have significant impacts on oocyst inactivation. When compared on the basis of germicidal UV dose, the oocysts were equally sensitive to low- and medium-pressure UV radiation. With respect to Cryptosporidium, both low- and medium-pressure ultraviolet radiation are attractive alternatives to conventional chemical disinfection methods in drinking water treatment.