DNA adducts in hematopoietic tissues and blood of the mummichog ( Fundulus heteroclitus) from a creosote-contaminated site in the Elizabeth River, Virginia

• Published in: Marine environmental research 2000-07, Vol.50 (1), p.581-589
• Main Authors: Rose, W.L, French, B.L, Reichert, W.L, Faisal, M
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Animal, plant and microbial ecology; Animals; Applied ecology; Benzopyrenes - metabolism; Biological and medical sciences; Creosote; Creosote - toxicity; DNA adducts; DNA Adducts - drug effects; DNA Adducts - metabolism; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Elizabeth River (Virginia); Fish; Freshwater; Fundamental and applied biological sciences. Psychology; Fundulus heteroclitus; Hematopoiesis - drug effects; Hematopoietic tissues; Image Processing, Computer-Assisted; Kidney - drug effects; Kidney - metabolism; Killifishes - blood; Liver - drug effects; Liver - metabolism; USA, Virginia; USA, Virginia, Elizabeth R; Virginia; Water Pollutants, Chemical - toxicity
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/S0141-1136(00)00252-X

Hydrophobic DNA adducts were examined in liver, anterior kidney, spleen, and blood of tumor-prone mummichog ( Fundulus heterclitus) from the creosote-contaminated Atlantic Wood (AW) site (Elizabeth River, Virginia). DNA adducts eluted in a diagonal radioactive zone, characteristic of polycyclic aromatic hydrocarbon exposure, in all examined tissues of AW fish. Mummichog demonstrated significantly higher levels of DNA adducts in spleen (394±109 nmol adducts/mol nucleotides) than in liver (201±77 nmol adducts/mol nucleotides) or anterior kidney (211±68 nmol adducts/mol nucleotides; P=0.036). The levels of DNA adducts in the pooled blood (pool of four) were 142 nmol adducts/mol nucleotides. DNA adducts were not detected in the liver, anterior kidney, spleen and blood of fish collected from the reference site (