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Investigation of simvastatin-induced apoptosis and cell cycle arrest in cancer stem cells of MCF-7

Context: Recent studies have shown the association between statins use and cancer risk reduction. Furthermore the importance of cancer stem cells (CSCs) in tumor initiation, progression and migration has been firmly established in a variety of solid tumors. Hence, the effective targeting of breast C...

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Published in:Journal of cancer research and therapeutics 2016-04, Vol.12 (2), p.725-730
Main Authors: Afzali, Monireh, Vatankhah, Melody, Ostad, Seyed
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creator Afzali, Monireh
Vatankhah, Melody
Ostad, Seyed
description Context: Recent studies have shown the association between statins use and cancer risk reduction. Furthermore the importance of cancer stem cells (CSCs) in tumor initiation, progression and migration has been firmly established in a variety of solid tumors. Hence, the effective targeting of breast CSCs has a potential to improve cancer treatment outcome significantly. Aims: This study has been designed to investigation the anticancer effects of simvastatin on breast CSCs. Settings and Design: In this study, MCF-7 CSCs were isolated from parent cells and cytotoxic effects of simvastatin were evaluated and compared in both cells. Subjects and Methods: Stem cell isolation was done by flow cytometry technique and the effects of simvastatin on the stem cell viability, apoptosis and cell cycle were evaluated and compared with parent cells. Statistical Analysis Used: The results were analyzed using one.way ANOVA, followed by Tukey.Kramer posttest. The P < 0.05 was considered as significant. Results: Based on the result, simvastatin shows dose-dependent cytotoxic effects on both CSCs and parent MCF-7 cells, whereas the apoptosis induction and the elimination of nonapoptotic programmed death were increased in CSC compared with parent cells. In addition, simvastatin showed the reduction in DNA synthesis and induced cell cycle arrest in the G1 phase in MCF-7 CSCs. Conclusions: This finding indicates that simvastatin with specific apoptotic effect on MCF-7 CSC may provide supporting reasons for future in vivo and in vitro statin trials.
doi_str_mv 10.4103/0973-1482.146127
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Furthermore the importance of cancer stem cells (CSCs) in tumor initiation, progression and migration has been firmly established in a variety of solid tumors. Hence, the effective targeting of breast CSCs has a potential to improve cancer treatment outcome significantly. Aims: This study has been designed to investigation the anticancer effects of simvastatin on breast CSCs. Settings and Design: In this study, MCF-7 CSCs were isolated from parent cells and cytotoxic effects of simvastatin were evaluated and compared in both cells. Subjects and Methods: Stem cell isolation was done by flow cytometry technique and the effects of simvastatin on the stem cell viability, apoptosis and cell cycle were evaluated and compared with parent cells. Statistical Analysis Used: The results were analyzed using one.way ANOVA, followed by Tukey.Kramer posttest. The P &lt; 0.05 was considered as significant. Results: Based on the result, simvastatin shows dose-dependent cytotoxic effects on both CSCs and parent MCF-7 cells, whereas the apoptosis induction and the elimination of nonapoptotic programmed death were increased in CSC compared with parent cells. In addition, simvastatin showed the reduction in DNA synthesis and induced cell cycle arrest in the G1 phase in MCF-7 CSCs. Conclusions: This finding indicates that simvastatin with specific apoptotic effect on MCF-7 CSC may provide supporting reasons for future in vivo and in vitro statin trials.</description><identifier>ISSN: 0973-1482</identifier><identifier>EISSN: 1998-4138</identifier><identifier>DOI: 10.4103/0973-1482.146127</identifier><identifier>PMID: 27461641</identifier><language>eng</language><publisher>India: Wolters Kluwer - Medknow Publications</publisher><subject>Antineoplastic Agents - pharmacology ; Apoptosis ; Apoptosis - drug effects ; Biomarkers ; Breast cancer ; Cancer cells ; Cancer therapies ; Cell cycle ; Cell Cycle Checkpoints - drug effects ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cytotoxicity ; Drug therapy ; Flow Cytometry ; Growth ; Health aspects ; Humans ; Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology ; Hypotheses ; Immunoglobulins ; Magnetic fields ; MCF-7 Cells ; Neoplastic Stem Cells - drug effects ; Neoplastic Stem Cells - metabolism ; Patient outcomes ; Population ; Simvastatin ; Simvastatin - pharmacology ; Statins ; Stem cells ; Studies ; Tumors</subject><ispartof>Journal of cancer research and therapeutics, 2016-04, Vol.12 (2), p.725-730</ispartof><rights>COPYRIGHT 2016 Medknow Publications and Media Pvt. 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Furthermore the importance of cancer stem cells (CSCs) in tumor initiation, progression and migration has been firmly established in a variety of solid tumors. Hence, the effective targeting of breast CSCs has a potential to improve cancer treatment outcome significantly. Aims: This study has been designed to investigation the anticancer effects of simvastatin on breast CSCs. Settings and Design: In this study, MCF-7 CSCs were isolated from parent cells and cytotoxic effects of simvastatin were evaluated and compared in both cells. Subjects and Methods: Stem cell isolation was done by flow cytometry technique and the effects of simvastatin on the stem cell viability, apoptosis and cell cycle were evaluated and compared with parent cells. Statistical Analysis Used: The results were analyzed using one.way ANOVA, followed by Tukey.Kramer posttest. The P &lt; 0.05 was considered as significant. 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identifier ISSN: 0973-1482
ispartof Journal of cancer research and therapeutics, 2016-04, Vol.12 (2), p.725-730
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source Publicly Available Content Database; IngentaConnect Journals
subjects Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Biomarkers
Breast cancer
Cancer cells
Cancer therapies
Cell cycle
Cell Cycle Checkpoints - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Cell Survival - drug effects
Cytotoxicity
Drug therapy
Flow Cytometry
Growth
Health aspects
Humans
Hydroxymethylglutaryl-CoA Reductase Inhibitors - pharmacology
Hypotheses
Immunoglobulins
Magnetic fields
MCF-7 Cells
Neoplastic Stem Cells - drug effects
Neoplastic Stem Cells - metabolism
Patient outcomes
Population
Simvastatin
Simvastatin - pharmacology
Statins
Stem cells
Studies
Tumors
title Investigation of simvastatin-induced apoptosis and cell cycle arrest in cancer stem cells of MCF-7
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