Mass spectrometry of gangliosides in extracranial tumors: Application to adrenal neuroblastoma

We report here on the introduction of mass spectrometry (MS) for profiling of native gangliosides from an extracranial tumor. The analytical approach was based on a modern platform combining the superior sensitivity and reproducibility of fully automated chip-based nanoelectrospray ionization (nanoE...

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Bibliographic Details
Published in:Analytical biochemistry 2016-09, Vol.509, p.1-11
Main Authors: Robu, Adrian C., Vukelić, Željka, Schiopu, Catalin, Capitan, Florina, Zamfir, Alina D.
Format: Article
Language:English
Subjects:
Adrenal Gland Neoplasms - metabolism
Chip–nanoelectrospray
Extracranial tumors
Fragmentation analysis
Gangliosides
Gangliosides - analysis
Gangliosides - metabolism
High-resolution mass spectrometry
Humans
Infant
Male
Mass Spectrometry - methods
Neuroblastoma
Neuroblastoma - metabolism
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Summary:We report here on the introduction of mass spectrometry (MS) for profiling of native gangliosides from an extracranial tumor. The analytical approach was based on a modern platform combining the superior sensitivity and reproducibility of fully automated chip-based nanoelectrospray ionization (nanoESI) with the high resolution and mass accuracy provided by a hybrid quadrupole time-of-flight (QTOF) instrument. The feasibility of the method for the analysis of gangliosides, which are much less expressed in extracranial tissues, was here tested using as the model substrate an adrenal neuroblastoma (NB) specimen located in the abdominal region of a 2-year-old infant. Under properly optimized conditions, MS profiling revealed information on at least 61 different gangliosides exhibiting heterogeneity of the glycan and lipid compositions. NB was found dominated by species bearing short-chain oligosaccharide cores with a reduced overall Neu5Ac content. By chip–nanoESI MS, preceding findings related to the GD2 role in NB were confirmed. Moreover, the screening experiments offered novel information supporting the possible biomarker role of GM4, GM3, and GM1 ganglioside classes. Structural analysis of GM1(d18:1/18:2) and GD1(d18:0/19:0) possibly tumor-associated markers, carried out by tandem MS (MS/MS) using collision-induced dissociation (CID) at low energies, indicated that both GM1a and GD1b isomers are present in NB. [Display omitted]
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2016.06.005