A method for easily customizable gradient gel electrophoresis

Gradient polyacrylamide gel electrophoresis is a powerful tool for the resolution of polypeptides by relative mobility. Here, we present a simplified method for generating polyacrylamide gradient gels for routine analysis without the need for specialized mixing equipment. The method allows for easil...

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Bibliographic Details
Published in:Analytical biochemistry 2016-09, Vol.509, p.12-14
Main Authors: Miller, Andrew J., Roman, Brandon, Norstrom, Eric
Format: Article
Language:English
Subjects:
Electrophoresis
Electrophoresis, Polyacrylamide Gel - methods
Gel
Glycine
Gradient
HEK293 Cells
Humans
Polyacrylamide
SDS-PAGE
Tricine
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Summary:Gradient polyacrylamide gel electrophoresis is a powerful tool for the resolution of polypeptides by relative mobility. Here, we present a simplified method for generating polyacrylamide gradient gels for routine analysis without the need for specialized mixing equipment. The method allows for easily customizable gradients which can be optimized for specific polypeptide resolution requirements. Moreover, the method eliminates the possibility of buffer cross contamination in mixing equipment, and the time and resources saved with this method in place of traditional gradient mixing, or the purchase of pre-cast gels, are noteworthy given the frequency with which many labs use gradient gel SDS-PAGE.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2016.07.003