Hydrogels containing porphyrin-loaded nanoparticles for topical photodynamic applications

[Display omitted] 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)-porphyrin tetra-iodide (TMPyP), a potent water-soluble photosensitizer (PS) used in antimicrobial applications, was encapsulated into poly(lactic-co-glycolic acid) (PLGA) nanoparticles (TMPyP-PLGA) for topical delivery purposes. Nanopart...

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Bibliographic Details
Published in:International journal of pharmaceutics 2016-08, Vol.510 (1), p.221-231
Main Authors: González-Delgado, José A., Castro, Pedro M., Machado, Alexandra, Araújo, Francisca, Rodrigues, Francisca, Korsak, Bárbara, Ferreira, Marta, Tomé, João P.C., Sarmento, Bruno
Format: Article
Language:English
Subjects:
Administration, Topical
Animals
Hydrogels
Hydrogels - administration & dosage
Hydrogels - chemistry
Nanoparticles - administration & dosage
Nanoparticles - chemistry
Photodynamic therapy
Photosensitizers
Photosensitizing Agents - administration & dosage
Photosensitizing Agents - chemistry
Polymeric nanoparticles
Porphyrins
Porphyrins - administration & dosage
Porphyrins - chemistry
Swine
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Summary:[Display omitted] 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)-porphyrin tetra-iodide (TMPyP), a potent water-soluble photosensitizer (PS) used in antimicrobial applications, was encapsulated into poly(lactic-co-glycolic acid) (PLGA) nanoparticles (TMPyP-PLGA) for topical delivery purposes. Nanoparticles resulted in a mean particle size around 130nm, narrow polydispersity index (PdI), spherical morphology and association efficiency up to 93%. Free TMPyP and TMPyP-PLGA nanoparticles were incorporated into Carbopol® hydrogels, resulting in controlled TMPyP release of about 60% and 20% after 4.5h, respectively. Critical properties such as appearance, clarity, viscosity and pH were maintained over time, as hydrogels were stable during 6 months at 4°C, 25°C/60% RH and 40°C/75% RH. For photodynamic applications, the photoproduction of singlet oxygen from these hydrogels was quite efficient being both formulations very photostable after 20min. No TMPyP permeation through pig ear skin was observed after 24h, and histological assays did not show relevant damages in surrounding tissues. All these excellent characteristics make them promising platforms for photodynamic applications through topical clinical use.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2016.06.037