Cinnamaldehyde derivatives inhibit degranulation and inflammatory mediator production in rat basophilic leukemia cells

• Published in: International immunopharmacology 2016-09, Vol.38, p.342-348
• Main Authors: Ahn, Sejin, Kim, Eunhee, Lee, Kooyeon, Lee, Deug-Chan
• Format: Article
• Language: English
• Subjects: 4-Chloro-cinnamaldehyde; 4-Trifluoro-cinnamaldehyde; Acrolein - analogs & derivatives; Acrolein - chemistry; Acrolein - pharmacology; Animals; Cell Degranulation - drug effects; Cell Line, Tumor; Cyclooxygenase 2 - genetics; Cyclooxygenase 2 - metabolism; Degranulation; Extracellular Signal-Regulated MAP Kinases - metabolism; Gene Expression Regulation - drug effects; Humans; Hypersensitivity - drug therapy; Hypersensitivity - immunology; Inflammation Mediators - metabolism; Interleukin-4 - genetics; Interleukin-4 - metabolism; Mast Cells - drug effects; Mast Cells - physiology; Mitogen-activated protein kinases; Rats; Signal Transduction - drug effects; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2016.06.018

Mast cells play a critical role in allergic diseases. Therefore, development of new therapeutic agents that suppresses the activation of mast cells may help prevent or treat allergic diseases. Here, we investigated the anti-allergic effects of 4-chloro-cinnamaldehyde and 4-trifluoro-cinnamaldehyde in RBL-2H3 cells. β-Hexosaminidase assays revealed that degranulation of RBL-2H3 cells was decreased following treatment with 60μM 4-chloro-cinnamaldehyde or 4-trifluoro-cinnamaldehyde. Moreover, quantitative real-time reverse transcription polymerase chain reaction showed that the relative expression levels of tumor necrosis factor-α, interleukin-4, and cyclooxygenase-2 mRNAs were decreased in RBL-2H3 cells treated with 4-chloro-cinnamaldehyde and 4-trifluoro-cinnamaldehyde in a concentration-dependent manner. Additionally, 4-chloro-cinnamaldehyde blocked the phosphorylation of MKKs and MAPKs. These data clearly suggested that 4-chloro-cinnamaldehyde and 4-trifluoro-cinnamaldehyde had inhibitory effects on the inflammatory responses of mast cells and may have potential as novel therapeutic agents for the prevention or treatment of allergic diseases. [Display omitted] •4-chloro-cinnamaldehyde and 4-trifluoro cinnamaldehyde inhibit degranulation of RBL-2H3 cells.•These compounds suppress the gene expression of proinflammatory cytokines and mediator like as TNF-α, IL-4 and COX-2.•These compounds suppress mast cell activation via inhibition of phosphorylation of MAPKs/MKKs signaling pathways in RBL-2H3 cells.