Inheritance of Anthracnose Resistance in the Common Bean Differential Cultivar G 2333 and Identification of a New Molecular Marker Linked to the Co-4 super(2) gene

• Published in: Journal of phytopathology 2001-05, Vol.149 (5), p.259-264
• Main Authors: Alzate-Marin, AL, Menarim, H, Soares Baia, G, Paula, TJ Jr, Almeida De Souza, K, Costa, Da, Goncalves De Barros, E, Moreira, MA
• Format: Article
• Language: English
• Subjects: Co-4@u2 gene; Colletotrichum lindemuthianum; Phaseolus vulgaris
• ISSN: 0931-1785

The inheritance of anthracnose resistance of the common bean (Phaseolus vulgaris L.) differential cultivar G 2333 to Colletotrichum lindemuthianum races 73 and 89 was studied in crosses with the susceptible cultivar Ruda. The segregation ratios of 15: 1 in the F sub(2) and 3: 1 in the backcrosses to Ruda indicate that for each of the races tested there are two independent resistance loci in G 2333. A random amplified polymorphic DNA (RAPD) molecular marker (OPH18 sub(1200C)) linked in resistance to race 73 was identified in a BC sub(3)F sub(2:3) population derived from crosses between Ruda and G 2333. A RAPD molecular marker OPAS13 sub(950C), previously identified as linked to gene Co-4 super(2), was also amplified in this population. Co-segregation analyses showed that these two markers are located at 5.6 (OPH18 sub(1200C)) and 11.2 (OPAS13 sub(950C)) cM of the Co-4 super(2) gene. These markers were not present in BC sub(1)F sub(2:3) plants resistant to race 89 indicating that this population carries a different resistance gene. DNA amplification of BC sub(1)F sub(2:3) plants with RAPD molecular marker OPAB sub(450C), previously identified as linked to gene Co-5, indicated that this gene is present in this population.