Sensitive detection of strong acidic condition by a novel rhodamine-based fluorescent pH chemosensor

A novel rhodamine‐based fluorescent pH probe responding to extremely low pH values has been synthesized and characterized. This probe showed an excellent photophysical response to pH on the basis that the colorless spirocyclic structure under basic conditions opened to a colored and highly fluoresce...

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Bibliographic Details
Published in:Luminescence (Chichester, England) England), 2016-05, Vol.31 (3), p.865-870
Main Authors: Tan, Jia-Lian, Yang, Ting-Ting, Liu, Yu, Zhang, Xue, Cheng, Shu-Jin, Zuo, Hua, He, Huawei
Format: Article
Language:English
Subjects:
Acids - analysis
Chemoreceptors
E. coli
Equilibrium equations
Escherichia coli
Escherichia coli - chemistry
extreme acidity
Extreme values
Fluorescence
Fluorescent Dyes - analysis
Fluorescent Dyes - chemistry
Hydrogen-Ion Concentration
Luminescence
Metal ions
rhodamine-derived probe
Rhodamines - analysis
Rhodamines - chemistry
Spectrophotometry
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Summary:A novel rhodamine‐based fluorescent pH probe responding to extremely low pH values has been synthesized and characterized. This probe showed an excellent photophysical response to pH on the basis that the colorless spirocyclic structure under basic conditions opened to a colored and highly fluorescent form under extreme acidity. The quantitative relationship between fluorescence intensity and pH value (1.75–2.62) was consistent with the equilibrium equation pH = pKa + log[(Imax – I)/(I – Imin)]. This sensitive pH probe was also characterized with good reversibility and no interaction with interfering metal ions, and was successfully applied to image Escherichia coli under strong acidity. Copyright © 2015 John Wiley & Sons, Ltd.
ISSN:1522-7235
1522-7243
DOI:10.1002/bio.3043