The Voltammetric Study of β–Glucosidase Hydrolytic Product with Modified Electrode

The novel modified electrode of voltammetric technique was applied to detect p-nitrophenol quantity released from the synthetic substrate p-nitrophenyl-β-D-glucopyranoside (pNPG) for β-glucosidase activity measurement. The results from the developed bismuth film modified screen printed carbon electr...

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Bibliographic Details
Published in:Key Engineering Materials 2016, Vol.675-676, p.671-674
Main Authors: Pitsanuwong, Chariwat, Wonsawat, Wanida, Charernchai, Sumamal
Format: Article
Language:English
Subjects:
Bismuth
Carbon
Electrodes
Enzyme activity
Enzymes
Purification
Screens
Seeds
Online Access:Get full text
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Summary:The novel modified electrode of voltammetric technique was applied to detect p-nitrophenol quantity released from the synthetic substrate p-nitrophenyl-β-D-glucopyranoside (pNPG) for β-glucosidase activity measurement. The results from the developed bismuth film modified screen printed carbon electrode (Bi/SPCE) for p-nitrophenol detecting, can be used for enzyme activity assay during purification steps. The further work, the condition of convenient voltammetric method will be optimized the condition suitable for applying to the β-glucosidase enzyme purification from Terminalia ivorensis C. seeds.
ISSN:1013-9826
1662-9795
1662-9795
DOI:10.4028/www.scientific.net/KEM.675-676.671