Influence of enzymatic hydrolysis on the biochemical methane potential of Chlorella vulgaris and Scenedesmus sp

BACKGROUND: The hard cell wall of some microalgae hampers efficient methane production when using those substrates. The present study investigated the effect of two groups of biocatalysts, namely carbohydrases and proteases, applied to Chlorella vulgaris and Scenedesmus sp. for microalgae hydrolysis...

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Published in:Journal of chemical technology and biotechnology (1986) 2016-05, Vol.91 (5), p.1299-1305
Main Authors: Mahdy, Ahmed, Mendez, Lara, Tomás-Pejó, Elia, del Mar Morales, Maria, Ballesteros, Mercedes, González-Fernández, Cristina
Format: Article
Language:English
Subjects:
Anaerobic digestion
carbohydrase
Carbohydrases
Carbohydrates
Chlorella vulgaris
Hydrolysis
Methane
Protease
Scenedesmus
Scenedesmus sp
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Summary:BACKGROUND: The hard cell wall of some microalgae hampers efficient methane production when using those substrates. The present study investigated the effect of two groups of biocatalysts, namely carbohydrases and proteases, applied to Chlorella vulgaris and Scenedesmus sp. for microalgae hydrolysis prior to anaerobic digestion. RESULTS: Chlorella vulgaris subjected to protease activity resulted in higher organic matter solubilisation (47%) than the carbohydrase treated samples (25–29%). Out of the carbohydrases tested, maximum carbohydrate solubilisation was reached by applying Viscozyme (84% for C. vulgaris and 36% for Scenedesmus sp.). The anaerobic digestion assays revealed that biomasses hydrolyzed with protease reached the highest methane yield. In spite of the different macromolecular composition and behavior towards the biocatalysts, protease hydrolysis before anaerobic digestion enhanced methane yield 1.72‐fold and 1.53‐fold for C. vulgaris and Scenedesmus sp., respectively. CONCLUSION: Despite the common belief that carbohydrates are responsible for the low digestibility of microalgae, this study suggested that proteins were the main polymers hampering anaerobic digestion. The addition of enzymes to enhance anaerobic digestibility could be questionable economically‐wise, however this investigation gives crucial information to elucidate the polymeric composition of two of the most common microalgae, C. vulgaris and Scenedesmus sp. © 2015 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.4722