Development and characterization of synthetic amphiploids of Oryza sativa and Oryza latifolia

Oryza sativa and Oryza latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, amphiploids were obtained from the tube seedlings of O. sativa × O. latifolia F1 hybrids by treatment with colchicine, an agent for chromosome doubling. Subsequently, amphiploids were investigat...

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Published in:Science bulletin (Beijing) 2015-12, Vol.60 (23), p.2059-2062
Main Authors: Yi, Chuandeng, Wang, Mingsen, Jiang, Wei, Wang, Derong, Cheng, Xiaojun, Wang, Ying, Zhou, Yong, Liang, Guohua, Gu, Minghong
Format: Article
Language:English
Subjects:
Amphiploid
anthers
Chemistry/Food Science
chromosome number
Chromosomes
Colchicine
Color
Deoxyribonucleic acid
Earth Sciences
Engineering
genetic markers
genome
Genomes
Genomic in situ hybridization
Humanities and Social Sciences
hybrids
in situ hybridization
inflorescences
Leaves
Letter
Life Sciences
Markers
metaphase
multidisciplinary
Oryza latifolia
Oryza sativa
Physics
root tips
Roots
Science
Science (multidisciplinary)
seedlings
Tubes
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cited_by cdi_FETCH-LOGICAL-c397t-9e3d69c05dcd1d43a1a3a910f6ecc5278b151077e2fe8ef3a6e249cb2c971acb3
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container_start_page 2059
container_title Science bulletin (Beijing)
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creator Yi, Chuandeng
Wang, Mingsen
Jiang, Wei
Wang, Derong
Cheng, Xiaojun
Wang, Ying
Zhou, Yong
Liang, Guohua
Gu, Minghong
description Oryza sativa and Oryza latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, amphiploids were obtained from the tube seedlings of O. sativa × O. latifolia F1 hybrids by treatment with colchicine, an agent for chromosome doubling. Subsequently, amphiploids were investigated using the methods of morphology, genomic in situ hybridization, and molecular markers. Amphiploids were characterized by a shorter plant height, larger diameter of stem, longer and wider leaves, darker leaf color, decreased spikelets per panicle and panicle length, and larger spikelets and anthers than the original F1 hybrid. Based on the mitotic metaphase chromosome number of the investigated root tips, the somatic chromosome number of the amphiploid is 2n = 72. Additionally, the amphiploid is an allohexaploid, and its genomic constitution is AACCDD by genomic in situ hybridization analysis. Finally, the amphiploids were identified to be true using 37 polymorphic markers at the DNA level.
doi_str_mv 10.1007/s11434-015-0944-3
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identifier ISSN: 2095-9273
ispartof Science bulletin (Beijing), 2015-12, Vol.60 (23), p.2059-2062
issn 2095-9273
2095-9281
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source SpringerLINK Contemporary 1997-Present; Elsevier
subjects Amphiploid
anthers
Chemistry/Food Science
chromosome number
Chromosomes
Colchicine
Color
Deoxyribonucleic acid
Earth Sciences
Engineering
genetic markers
genome
Genomes
Genomic in situ hybridization
Humanities and Social Sciences
hybrids
in situ hybridization
inflorescences
Leaves
Letter
Life Sciences
Markers
metaphase
multidisciplinary
Oryza latifolia
Oryza sativa
Physics
root tips
Roots
Science
Science (multidisciplinary)
seedlings
Tubes
title Development and characterization of synthetic amphiploids of Oryza sativa and Oryza latifolia
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