Mid-infrared spectroscopy for protein analysis: potential and challenges

Mid-infrared (MIR) spectroscopy investigates the interaction of MIR photons with both organic and inorganic molecules via the excitation of vibrational and rotational modes, providing inherent molecular selectivity. In general, infrared (IR) spectroscopy is particularly sensitive to protein structur...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2016-04, Vol.408 (11), p.2875-2889
Main Authors: López-Lorente, Ángela I., Mizaikoff, Boris
Format: Article
Language:English
Subjects:
Analytical Chemistry
Backbone
Biochemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Circular Dichroism
Food Science
Infrared spectroscopy
Laboratory Medicine
Methods
Molecular structure
Monitoring/Environmental Analysis
Polypeptides
Protein research
Proteins
Proteins - analysis
Review
Spectrophotometry, Infrared - methods
Spectroscopy
Young Investigators in Analytical and Bioanalytical Science
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Summary:Mid-infrared (MIR) spectroscopy investigates the interaction of MIR photons with both organic and inorganic molecules via the excitation of vibrational and rotational modes, providing inherent molecular selectivity. In general, infrared (IR) spectroscopy is particularly sensitive to protein structure and structural changes via vibrational resonances originating from the polypeptide backbone or side chains; hence information on the secondary structure of proteins can be obtained in a label-free fashion. In this review, the challenges for IR spectroscopy for protein analysis are discussed as are the potential and limitations of different IR spectroscopic techniques enabling protein analysis. In particular, the amide I spectral range has been widely used to study protein secondary structure, conformational changes, protein aggregation, protein adsorption, and the formation of amyloid fibrils. In addition to representative examples of the potential of IR spectroscopy in various fields related to protein analysis, the potential of protein analysis taking advantage of miniaturized MIR systems, including waveguide-enhanced MIR sensors, is detailed. Graphical Abstract A diagnosis of the potential and challenges of mid-infrared spectroscopy for protein analysis is given in this review
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-016-9375-5