Influence of oxidative injury and monitoring of blood plasma by DSC on patients with psoriasis

• Published in: Journal of thermal analysis and calorimetry 2016-03, Vol.123 (3), p.2037-2043
• Main Authors: Moezzi, Medhi, Zapf, István, Fekecs, Tamás, Nedvig, Klára, Lőrinczy, Dénes, Ferencz, Andrea
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Antioxidants; Antioxidants (Nutrients); Blood plasma; Calorimetry; Care and treatment; Chemistry; Chemistry and Materials Science; Differential scanning calorimetry; Drugs; Inorganic Chemistry; Measurement Science and Instrumentation; Methotrexate; Oxidative stress; Patients; Physical Chemistry; Polymer Sciences; Protein denaturation; Psoriasis; Retinoids; Stress concentration; Superoxides; Therapy
• ISSN: 1388-6150; 1588-2926; 1572-8943
• DOI: 10.1007/s10973-015-4674-1

Oxidative stress induced by oxygen free radicals (OFRs) is a casual factor in psoriasis. Our aim was to detect the oxidative stress parameters and blood plasma changes with differential scanning calorimetry (DSC) in psoriatic patients. The study included untreated ( n  = 39) and treated (retinoids, methotrexate, biologic response modifiers; n  = 33) white adult patients from both sex. To monitor oxidative stress concentration of malondialdehyde (MDA), reduced glutathione and sulfhydryl groups, production of OFRs, and activity of myeloperoxidase (MPO), superoxide dismutase, and catalase were measured. Denaturation of plasma components was detected in SETARAM Micro DSC-II calorimeter. Total production of OFRs and MPO activity, and the concentration of MDA were significantly increased both in untreated patients with moderate and severe symptoms and in all drug-treated groups compared with controls ( p