Importance of the hydrogen route in up-scaling electrosynthesis for microbial CO sub(2) reduction

• Published in: Energy & environmental science 2015-11, Vol.8 (12), p.3731-3744
• Main Authors: Blanchet, Elise, Duquenne, Francois, Rafrafi, Yan, Etcheverry, Luc, Erable, Benjamin, Bergel, Alain
• Format: Article
• Language: English
• Subjects: Acetates; Carbon dioxide; Formates; Hydrogen; Inoculum; Microorganisms; Reduction; Reduction (electrolytic); Sporomusa
• ISSN: 1754-5692; 1754-5706
• DOI: 10.1039/c5ee03088a

Microbial electrochemical reduction of CO sub(2) was carried out under two different applied potentials, -0.36 V and -0.66 V vs.SHE, using a biological sludge as the inoculum. Both potentials were thermodynamically appropriate for converting CO sub(2) to acetate but only -0.66 V enabled hydrogen evolution. No acetate production was observed at -0.36 V, while up to 244 plus or minus 20 mg L super(-1) acetate was produced at -0.66 V vs.SHE. The same microbial inoculum implemented in gas-liquid contactors with H sub(2) and CO sub(2) gas supply led to acetate production of 2500 mg L super(-1). When a salt marsh sediment was used as the inoculum, no reduction was observed in the electrochemical reactors, while supplying H sub(2) + CO sub(2) gas led to formate and then acetate production. Finally, pure cultures of Sporomusa ovatagrown under H sub(2) and CO sub(2) gas feeding showed acetate production of up to 2904 mg L super(-1), higher than those reported so far in the literature for S. ovataimplemented in bioelectrochemical processes. Unexpected ethanol production of up to 1411 mg L super(-1) was also observed. All these experimental data confirm that hydrogen produced on the cathode by water electrolysis is an essential mediator in the microbial electrochemical reduction of CO sub(2). Implementing homoacetogenic microbial species in purposely designed gas-liquid biocontactors should now be considered as a relevant strategy for developing CO sub(2) conversion.