Selective ciprofloxacin antibiotic detection by fluorescent siderophore pyoverdin

Fluorescent siderophore pyoverdin (PVD) was produced from a soil isolate Pseudomonas monteilii strain MKP 213. The PVD was purified near to homogeneity and applied for the fluorescent chemosensing of various antibiotics in aqueous solution (pH=7.0). Upon addition of ciprofloxacin, PVD showed new UV–...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2016-07, Vol.81, p.274-279
Main Authors: Pawar, Madhuri K., Tayade, Kundan C., Sahoo, Suban K., Mahulikar, Pramod P., Kuwar, Anil S., Chaudhari, Bhushan L.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - analysis
Antibiotics
Antibiotics sensing
Biosensors
Ciprofloxacin
Ciprofloxacin - analysis
Constants
Fluorescence
Fluorescent Dyes - chemistry
Fluorescent siderophore
Homogeneity
Humans
Limit of Detection
MCF-7 Cells
Oligopeptides - chemistry
Peptides
Physical vapor deposition
Pseudomonas
Pseudomonas - chemistry
Pyoverdin
Siderophores - chemistry
Spectrometry, Fluorescence - methods
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Summary:Fluorescent siderophore pyoverdin (PVD) was produced from a soil isolate Pseudomonas monteilii strain MKP 213. The PVD was purified near to homogeneity and applied for the fluorescent chemosensing of various antibiotics in aqueous solution (pH=7.0). Upon addition of ciprofloxacin, PVD showed new UV–vis absorption bands at 252 and 321nm due to an internal charge transfer mechanism. Also, the addition of ciprofloxacin induced a highly selective fluorescence enhancement of PVD with a 13nm blue shift from 458 to 445nm. The combination of a long peptide chain along with the chromophore unit of PVD generates a converging cleft for ciprofloxacin recognition with LOD and LOQ of 7.13μM and 21.6μM, respectively without interference from other studied antibiotics. The association constant (Ka) of PVD with ciprofloxacin was calculated to be as low as 1.40×105M−1 using Benesi-Hildebrand plot depicting its significance in detection. The pharmaceutical tablet analysis measures the sensing with negligible matrix effect and quantitative recovery. •Pyoverdin (PVD) from Pseudomonas monteilii strain MKP 213 was purified and identified.•A highly selective fluorescent siderophore PVD based off–on fluorescence chemosensor for ciprofloxacin is reported.•Ciprofloxacin sensed with micromolar detection limit (as low as 7.1µM) is reported.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2016.03.003