Fluorescence Enhancement at Docking Sites of DNA-Directed Self-Assembled Nanoantennas

We introduce self-assembled nanoantennas to enhance the fluorescence intensity in a plasmonic hotspot of zeptoliter volume. The nanoantennas are prepared by attaching one or two gold nanoparticles (NPs) to DNA origami structures, which also incorporated docking sites for a single fluorescent dye nex...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2012-10, Vol.338 (6106), p.506-510
Main Authors: Acuna, G. P., Möller, F. M., Holzmeister, P., Beater, S., Lalkens, B., Tinnefeld, P.
Format: Article
Language:English
Subjects:
Antennas
Binding sites
Condensed matter: electronic structure, electrical, magnetic, and optical properties
Deoxyribonucleic acid
Dimerization
Dimers
DNA
DNA - chemistry
DNA - genetics
DNA, Cruciform
Docking
Dyes
Electric fields
Electronic structure and electrical properties of surfaces, interfaces, thin films and low-dimensional structures
Electronic structure of nanoscale materials : clusters, nanoparticles, nanotubes, and nanocrystals
Emission
Exact sciences and technology
Fluorescence
Fluorescent Dyes - chemistry
Gold
Gold - chemistry
Image Enhancement - methods
Metal Nanoparticles - chemistry
Molecular biology
Molecules
Monomers
Nanoparticles
Nanostructure
Origami
Particle Size
Physics
Plasmonics
Pollutant emissions
Rotation
Spectrometry, Fluorescence - methods
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Summary:We introduce self-assembled nanoantennas to enhance the fluorescence intensity in a plasmonic hotspot of zeptoliter volume. The nanoantennas are prepared by attaching one or two gold nanoparticles (NPs) to DNA origami structures, which also incorporated docking sites for a single fluorescent dye next to one NP or in the gap between two NPs. We measured the dependence of the fluorescence enhancement on NP size and number and compare it to numerical simulations. A maximum of 117-fold fluorescence enhancement was obtained for a dye molecule positioned in the 23-nanometer gap between 100-nanometer gold NPs. Direct visualization of the binding and unbinding of short DNA strands, as well as the conformational dynamics of a DNA Holliday junction in the hotspot of the nanoantenna, show the compatibility with single-molecule assays.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1228638