Liver and kidney cells cultures in a new perfluoropolyether biochip

•Fluorinated microfluidic biochips were developed from perfluoropolyethers (PFPEs).•All devices showed a good patterns and efficient sealing.•Adhesion and growth of cells in PFPEs biochips were similar to PDMS biochips.•Cells metabolism was maintained during culture in dynamic biochips. Although mic...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2016-06, Vol.229, p.396-407
Main Authors: Jellali, Rachid, Paullier, Patrick, Fleury, Marie-José, Leclerc, Eric
Format: Article
Language:English
Subjects:
Biochips
Cell growth
Construction
Devices
Kidney
Kidneys
Liver
Metabolism
Microfluidic biochips
Microfluidics
Perfluoropolyethers
Silicone resins
Tricalcium aluminum ferrite
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Summary:•Fluorinated microfluidic biochips were developed from perfluoropolyethers (PFPEs).•All devices showed a good patterns and efficient sealing.•Adhesion and growth of cells in PFPEs biochips were similar to PDMS biochips.•Cells metabolism was maintained during culture in dynamic biochips. Although microfluidics represents a promising technology for drug screening industry and toxicity tests, their industrial applications using cells are limited by drawbacks of the weakly mass production capability of the biochips. In this work, we report the fabrication of resistant fluorinated microfluidic devices using a material widely used in polymer industries. To build the microdevices, two patterned layers with precise and regular microchannels were developed by photocuring of perfluoropolyethers (PFPEs). These layers were successfully sealed by UV irradiation. Then, Liver HepG2/C3A and kidney MDCK cells were cultured in PFPE biochips. The growth, cell viability and basal metabolism of cells cultured in PFPE biochips were studied and compared with results obtained using polydimethylsiloxane (PDMS) biochips. The results have shown that the cells can attach to the biochip bottom, spread, and proliferate well in PFPE biochip (similar to the cells in PDMS biochip). Furthermore, metabolisms of cell cultures in PFPE biochip, such as glucose consumption, albumin and urea productions, were proved similar to the results obtained in PDMS biochips. These results highlighted the functionality of the HepG2/C3A and MDCK cells in PFPE microfluidic devices and illustrated their potential to replace PDMS devices.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2016.01.141