Isolation, structural and functional characterization of a new Lys49 phospholipase A2 homologue from Bothrops neuwiedi urutu with bactericidal potential

• Published in: Toxicon (Oxford) 2016-06, Vol.115, p.13-21
• Main Authors: Corrêa, Edailson A., Kayano, Anderson M., Diniz-Sousa, Rafaela, Setúbal, Sulamita S., Zanchi, Fernando B., Zuliani, Juliana P., Matos, Najla B., Almeida, José R., Resende, Letícia M., Marangoni, Sérgio, da Silva, Saulo L., Soares, Andreimar M., Calderon, Leonardo A.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bacteria; Bactericidal activity; Bothrops; Bothrops - metabolism; Bothrops neuwiedi; Cell Survival - drug effects; Crotalid Venoms - chemistry; Crotalid Venoms - isolation & purification; Crotalid Venoms - pharmacology; Edema - blood; Edema - chemically induced; Edema - pathology; Electrophoresis, Polyacrylamide Gel; Fragments; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; Homology; Interleukin-1beta - blood; Male; Mice; Peptides; Phospholipase; Phospholipase A2; Phospholipases A2 - chemistry; Phospholipases A2 - pharmacology; Protein Conformation; Proteins; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Sequence Alignment; Snake venom; Snakes; Tumor Necrosis Factor-alpha - blood
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2016.02.021

Snake venom is a complex mixture of active compounds consisting of 80–90% proteins and peptides that exhibit a variety of biological actions that are not completely clarified or identified. Of these, phospholipase A2 is one of the molecules that has shown great biotechnological potential. The objectives of this study were to isolate, biochemically and biologically characterize a Lys49 phospholipase A2 homologue from the venom of Bothrops neuwiedi urutu. The protein was purified after two chromatographic steps, anion exchange and reverse phase. The purity and relative molecular mass were assessed by SDS-PAGE, observing a molecular weight typical of PLA2s, subsequently confirmed by mass spectrometry obtaining a mass of 13,733 Da. As for phospholipase activity, the PLA2 proved to be enzymatically inactive. The analyses by Edman degradation and sequencing of the peptide fragments allowed for the identification of 108 amino acid residues; this sequence showed high identity with other phospholipases A2 from Bothrops snake venoms, and identified this molecule as a novel PLA2 isoform from B. neuwiedi urutu venom, called BnuTX-I. In murine models, both BnuTX-I as well as the venom induced edema and myotoxic responses. The cytotoxic effect of BnuTX-I in murine macrophages was observed at concentrations above 12 μg/mL. BnuTX-I also presented antimicrobial activity against gram-positive and negative bacterial strains, having the greatest inhibitory effect on Pseudomonas aeruginosa. The results allowed for the identification of a new myotoxin isoform with PLA2 structure with promising biotechnological applications. [Display omitted] •New Lys49 Phospholipase A2 Homologue from Bothrops neuwiedi urutu.•BnuTX-I, PLA2 enzymatically inactive with Bactericidal Potential.•BnuTX-I presented antimicrobial activity, greatest inhibitory effect on Pseudomonas aeruginosa.