Anthocyanin-rich extracts from blackberry, wild blueberry, strawberry, and chokeberry: antioxidant activity and inhibitory effect on oleic acid-induced hepatic steatosis in vitro

• Published in: Journal of the science of food and agriculture 2016-05, Vol.96 (7), p.2494-2503
• Main Authors: Wang, Yong, Zhao, Liang, Wang, Dan, Huo, Yazhen, Ji, Baoping
• Format: Article
• Language: English
• Subjects: Animals; Anthocyanins - chemistry; Antioxidants; Antioxidants - chemistry; Berries; Blueberries; Blueberry Plants - chemistry; Clearances; cyanidin-3-glucoside; Fatty Liver - chemically induced; Fatty Liver - prevention & control; Food science; Fragaria; Fragaria - chemistry; Fruit - chemistry; Hep G2 Cells; Humans; In vitro testing; Lipid Metabolism - drug effects; non-alcoholic fatty liver disease; Oleic Acid - toxicity; Oxygen; Plant Extracts - chemistry; Plant Extracts - pharmacology; Prunus - chemistry; Radicals; reactive oxygen species; Rings (mathematics); Rubus - chemistry; Strawberries; triglyceride; Vaccinium
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.7370

BACKGROUND Limited information is available regarding the relationship between the chemical structures and inhibitory effects of anthocyanin (ACN) on triglyceride (TG) overaccumulation. Thus this study investigated the antioxidant activity and inhibitory effect of blackberry, wild blueberry, strawberry, and chokeberry ACN‐rich extracts, with different structural characteristics, on oleic acid‐induced hepatic steatosis in vitro. Four major ACNs from these berries, with different aglycones, namely cyanidin‐3‐glucoside (Cy‐3‐glu), delphinidin‐3‐glucoside, pelargonidin‐3‐glucoside, and malvidin‐3‐glucoside, were also investigated. RESULTS Blackberry ACN‐rich extract exhibited the most significant inhibitory effect on TG clearance (30.5% ± 3.4%) and reactive oxygen species generation. TG clearance was significantly correlated with total phenolic content (r = 0.991, P