Separation of three phenolic high-molecular-weight compounds from the crude extract of Terminalia Chebula Retz. by ultrasound-assisted extraction and high-speed counter-current chromatography

This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound‐assisted extraction and high‐speed counter‐current chromatography. The ultrasound‐assisted extraction conditions were optim...

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Bibliographic Details
Published in:Journal of separation science 2016-04, Vol.39 (7), p.1278-1285
Main Authors: Zou, Deng-lang, Chen, Tao, Li, Hong-mei, Chen, Chen, Zhao, Jing-yang, Li, Yu-lin
Format: Article
Language:English
Subjects:
Chromatography
Countercurrent Distribution
Crude extracts
Extraction
Herbal medicine
High speed
High-speed counter-current chromatography
Methyl alcohol
Molecular Weight
NMR spectroscopy
Phenolic compounds
Phenols
Phenols - chemistry
Phenols - isolation & purification
Plant Extracts - chemistry
Response surface methodology
Separation
Strategy
Terminalia
Terminalia - chemistry
Terminalia chebula Retz
Ultrasonic imaging
Ultrasonics
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Summary:This study presents an efficient strategy for separation of three phenolic compounds with high molecular weight from the crude extract of Terminalia chebula Retz. by ultrasound‐assisted extraction and high‐speed counter‐current chromatography. The ultrasound‐assisted extraction conditions were optimized by response surface methodology and the results showed the target compounds could be well enriched under the optimized extraction conditions. Then the crude extract was directly separated by high‐speed counter‐current chromatography without any pretreatment using n‐hexane/ethyl acetate/methanol/water (1:7:0.5:3, v/v/v/v) as the solvent system. In 180 min, 13 mg of A, 18 mg of B, and 9 mg of C were obtained from 200 mg of crude sample. Their structures were identified as Chebulagic acid (A, 954 Da), Chebulinic acid (B, 956 Da), and Ellagic acid (C) by 1H NMR spectroscopy.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201501358