Lack of effect of beta-naphthoflavone on induction of Nramp genes in adult rainbow trout Oncorhynchus mykiss

• Published in: Marine environmental research 2000-07, Vol.50 (1), p.147-151
• Main Authors: Dasmahapatra, A.K, Wimpee, B.A.B, Budsberg, K.J, Dorschner, M.O, Phillips, R.B, Hutz, R.J
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Animal, plant and microbial ecology; Animals; Applied ecology; aromatic hydrocarbons; B-naphthoflavone; beta-Naphthoflavone - toxicity; Biological and medical sciences; BNF; Carrier Proteins - genetics; Cation Transport Proteins; CYP1A1; Cytochrome P-450 CYP1A1 - biosynthesis; Cytochrome P-450 CYP1A1 - metabolism; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on vertebrates; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Iron-Binding Proteins; Kidney - drug effects; Kidney - metabolism; Male; Marine; Membrane Proteins - genetics; mRNA; natural resistance-associated macrophage protein; Nramp; Nramp gene; Oncorhynchus mykiss; Oncorhynchus mykiss - genetics; Oncorhynchus mykiss - metabolism; Ovary; Ovary - drug effects; Ovary - metabolism; Rainbow trout; Receptors, Aryl Hydrocarbon - metabolism; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA, Messenger - metabolism; RT-PCR; Water Pollutants, Chemical - toxicity
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/S0141-1136(00)00113-6

Natural resistance-associated macrophage protein (Nramp) genes in rainbow trout, Oncorhynchus mykiss, were identified and characterized. The greatest mRNA level encoding these genes was in the developing ovary of rainbow trout. We evaluated the response of these genes to a certain aromatic hydrocarbon receptor (AHR) agonist. Adult rainbow trout were treated with β-naphthoflavone (BNF) (50 and 100 mg/kg) for 48 h. Using reverse-transcriptase polymerase chain reaction with ovary and head kidney RNA and specific α and β Nramp primers, a 400 bp Nramp-α- and a 400 bp Nramp-β-specific cDNA were obtained. There were no changes in the α and β Nramp mRNA levels in the ovary following BNF administration. CYP1A1 mRNA was increased in the ovary and kidney, suggesting the presence of AHR in rainbow trout ovary, while the AHR agonist produced no effect on Nramp mRNAs.