HSP27 modulates survival signaling in endosulfan-exposed human peripheral blood mononuclear cells treated with curcumin

Endosulfan, a well-known organochlorine pesticide, induces apoptosis and depletion of reduced glutathione (GSH) in human peripheral blood mononuclear cells (PBMC). Thus, for the amelioration of its effect, antioxidant and antiapoptotic potential of curcumin was evaluated. For ascertaining the attenu...

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Bibliographic Details
Published in:Human & experimental toxicology 2016-07, Vol.35 (7), p.695-704
Main Authors: Ahmed, T, Banerjee, BD
Format: Article
Language:English
Subjects:
Antioxidants
Apoptosis
Apoptosis - drug effects
Attenuation
Biochemistry
Cell Survival - drug effects
Cells, Cultured
Curcumin
Curcumin - pharmacology
Cytochrome
Cytochrome c
Cytochromes c - metabolism
Cytotoxicity
Damage
Deoxyribonucleic acid
DNA
DNA fragmentation
Dose-Response Relationship, Drug
Endosulfan
Endosulfan - toxicity
Exposure
Fragmentation
Glutathione
HSP27 Heat-Shock Proteins - metabolism
Hsp27 protein
Humans
Immunotoxicity
Leukocytes (mononuclear)
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - metabolism
Leukocytes, Mononuclear - pathology
Microscopy, Fluorescence
Organic compounds
Organochlorine pesticides
Oxidative stress
Peripheral blood mononuclear cells
Phosphatidylserine
Restoration
Survival
Toxicity
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Summary:Endosulfan, a well-known organochlorine pesticide, induces apoptosis and depletion of reduced glutathione (GSH) in human peripheral blood mononuclear cells (PBMC). Thus, for the amelioration of its effect, antioxidant and antiapoptotic potential of curcumin was evaluated. For ascertaining the attenuating effect of curcumin, various biochemical indices of cell damage such as cytotoxicity, oxidative stress, apoptosis (phosphatidylserine externalization, DNA fragmentation, and cytochrome c) in human PBMC was evaluated following endosulfan exposure (0–100 µM). To assess the role of HSP27 on endosulfan-induced apoptosis, the expression of HSP27 was examined. Curcumin (25 µM) increased cell viability significantly. As evident from the restoration of GSH, antiapoptotic potential was directly proportional to their antioxidant nature of curcumin. The present study indicates that the beneficial effect of curcumin on endosulfan-induced cytotoxicity is related to the induced synthesis of HSP27, emphasizing its antioxidant and therapeutic potential as well as underscoring the mechanism of pesticide-induced toxicity at cellular level. Taken together, these findings suggest that curcumin protects against endosulfan-induced immunotoxicity in human PBMC by attenuating apoptosis.
ISSN:0960-3271
1477-0903
DOI:10.1177/0960327115597986