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Monitoring and cell-specific deletion of C5aR1 using a novel floxed GFP-C5aR1 reporter knock-in mouse

Many of the biological properties of C5a are mediated through activation of its receptor (C5aR1), the expression of which has been demonstrated convincingly on myeloid cells, such as neutrophils, monocytes, and macrophages. In contrast, conflicting results exist regarding C5aR1 expression in dendrit...

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Published in:The Journal of immunology (1950) 2015-02, Vol.194 (4), p.1841-1855
Main Authors: Karsten, Christian M, Laumonnier, Yves, Eurich, Benjamin, Ender, Fanny, Bröker, Katharina, Roy, Sreeja, Czabanska, Anna, Vollbrandt, Tillman, Figge, Julia, Köhl, Jörg
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cited_by cdi_FETCH-LOGICAL-c374t-34ee575e0b773043586723348b180195a8d3a45df672edfdc5866f6a966acea3
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container_issue 4
container_start_page 1841
container_title The Journal of immunology (1950)
container_volume 194
creator Karsten, Christian M
Laumonnier, Yves
Eurich, Benjamin
Ender, Fanny
Bröker, Katharina
Roy, Sreeja
Czabanska, Anna
Vollbrandt, Tillman
Figge, Julia
Köhl, Jörg
description Many of the biological properties of C5a are mediated through activation of its receptor (C5aR1), the expression of which has been demonstrated convincingly on myeloid cells, such as neutrophils, monocytes, and macrophages. In contrast, conflicting results exist regarding C5aR1 expression in dendritic cells (DCs) and lymphoid lineage cells. In this article, we report the generation of a floxed GFP-C5aR1 reporter knock-in mouse. Using this mouse strain, we confirmed strong C5aR1 expression in neutrophils from bone marrow, blood, lung, and spleen, as well as in peritoneal macrophages. Further, we show C5aR1 expression in lung eosinophils, lung- and lamina propria-resident and alveolar macrophages, bone marrow-derived DCs, and lung-resident CD11b(+) and monocyte-derived DCs, whereas intestinal and pulmonary CD103(+) DCs stained negative. Also, some splenic NKT cells expressed GFP, whereas naive NK cells and B2 cells lacked GFP expression. Finally, we did not observe any C5aR1 expression in naive or activated CD4(+) Th cells in vitro or in vivo. Mating the floxed GFP-C5aR1 mouse strain with LysMCre mice, we were able to specifically delete C5aR1 in neutrophils and macrophages, whereas C5aR1 expression was retained in DCs. In summary, our findings suggest that C5aR1 expression in mice is largely restricted to cells of the myeloid lineage. The novel floxed C5aR1 reporter knock-in mouse will prove useful to track C5aR1 expression in experimental models of acute and chronic inflammation and to conditionally delete C5aR1 in immune cells.
doi_str_mv 10.4049/jimmunol.1401401
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Mating the floxed GFP-C5aR1 mouse strain with LysMCre mice, we were able to specifically delete C5aR1 in neutrophils and macrophages, whereas C5aR1 expression was retained in DCs. In summary, our findings suggest that C5aR1 expression in mice is largely restricted to cells of the myeloid lineage. 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subjects Animals
Cell Separation
Flow Cytometry
Gene Knock-In Techniques
Genes, Reporter
Green Fluorescent Proteins - genetics
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Myeloid Cells - immunology
Myeloid Cells - metabolism
Real-Time Polymerase Chain Reaction
Receptor, Anaphylatoxin C5a - analysis
Receptor, Anaphylatoxin C5a - biosynthesis
Receptor, Anaphylatoxin C5a - immunology
title Monitoring and cell-specific deletion of C5aR1 using a novel floxed GFP-C5aR1 reporter knock-in mouse
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