Rabies direct fluorescent antibody test does not inactivate rabies or eastern equine encephalitis viruses

• Published in: Journal of virological methods 2016-08, Vol.234, p.52-53
• Main Authors: Jarvis, Jodie A., Franke, Mary A., Davis, April D.
• Format: Article
• Language: English
• Subjects: Acetone; Acetone - chemistry; Acetone - pharmacology; Animals; Antibodies, Viral - chemistry; Antibodies, Viral - immunology; Brain - virology; Diagnosis; Direct fluorescent antibody test; Eastern equine encephalitis; Encephalitis Virus, Eastern Equine - immunology; Encephalitis Virus, Eastern Equine - physiology; Encephalomyelitis, Eastern Equine - diagnosis; Encephalomyelitis, Eastern Equine - immunology; Encephalomyelitis, Eastern Equine - veterinary; Encephalomyelitis, Eastern Equine - virology; Fluorescent Antibody Technique, Direct; Histological Techniques - methods; Horses; Humans; Inactivation; Rabies; Rabies - veterinary; Rabies virus - immunology; Rabies virus - physiology; Staining and Labeling - methods; Staining and Labeling - standards; Virus Inactivation
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2016.04.005

•Acetone fixation and the routine DFA test do not inactivate RV or EEEV.•Published inactivation techniques for RV or EEEV are not practical for routine tests.•All tissue samples should be treated as infectious throughout the whole procedure. An examination using the routine rabies direct fluorescent antibody test was performed on rabies or Eastern equine encephalitis positive mammalian brain tissue to assess inactivation of the virus. Neither virus was inactivated with acetone fixation nor the routine test, thus laboratory employees should treat all samples as rabies and when appropriate Eastern equine encephalitis positive throughout the whole procedure.