Freeze-dried egg yolk based extenders containing various antioxidants improve post-thawing quality and incubation resilience of goat spermatozoa

The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in cont...

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Bibliographic Details
Published in:Cryobiology 2016-06, Vol.72 (3), p.269-273
Main Authors: Alcay, Selim, Gokce, Elif, Toker, M. Berk, Onder, N. Tekin, Ustuner, Burcu, Uzabacı, Ender, Gul, Zulfiye, Cavus, Seda
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Antioxidants - pharmacology
Butylated Hydroxytoluene - pharmacology
Cell Membrane - drug effects
Cryopreservation
Cryopreservation - methods
Cysteamine - pharmacology
Egg Yolk
Freeze-drying
Goat semen
Goats
In Situ Nick-End Labeling
Incubation resilience
Male
Malondialdehyde - metabolism
Semen
Semen Preservation - methods
Sperm Motility
Spermatozoa
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Summary:The aim of this study was to evaluate different antioxidants-supplemented freeze-dried egg yolk based extenders for the post-thawing quality and incubation resilience of goat spermatozoa. Pooled semen were diluted in a two-step dilution method to a final concentration of 1/5 (semen/extender) in control and antoxidant supplemented freeze-dried extenders (methionine, cysteamine and butylated hydroxytoluene). Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypoosmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) and DNA integrity using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Membrane lipid peroxidation status was also analyzed using the malondialdehyde (MDA) concentration. In the study, antioxidant supplemented freeze-dried egg yolk based extenders have beneficial effect on goat sperm parameters. In addition, we achieved a higher quality in post thawed goat semen even after 6 h incubation when the extender was supplemented by 5 mM BHT or cysteamine.
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2016.03.007