Studies on pathology, cytokine gene expression and molecular typing of Mycobacterium avium subsp. paratuberculosis of naturally occurring Johne's disease in bullocks

Pathology of Johne's disease (JD) in bullocks (castrated, adult male cattle) is rarely studied. Here, we report the pathology and cytokine gene expression of naturally occurring JD in bullocks. The small intestine and mesenteric lymph nodes collected from 404 bullocks, aged between 5 and 10year...

Full description

Saved in:
Bibliographic Details
Published in:Research in veterinary science 2016-06, Vol.106, p.74-80
Main Authors: Narnaware, S.D., Periasamy, S., Tripathi, B.N.
Format: Article
Language:English
Subjects:
Animals
Bison
Bullocks
Cattle
Cattle Diseases - microbiology
Cytokines
Cytokines - genetics
Cytokines - metabolism
Deoxyribonucleic acid
DNA
Gene expression
Gene Expression Regulation, Bacterial
IFNγ
IL-10
IL-1α
Immunohistochemistry
IS1311 PCR REA
IS900
Johne's disease
Laboratories
Male
Molecular Typing
Mycobacterium avium
Mycobacterium avium subsp. paratuberculosis - genetics
Mycobacterium avium subsp. paratuberculosis - metabolism
Paratuberculosis - microbiology
Pathology
Small intestine
Studies
TGFβ
Veterinary medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pathology of Johne's disease (JD) in bullocks (castrated, adult male cattle) is rarely studied. Here, we report the pathology and cytokine gene expression of naturally occurring JD in bullocks. The small intestine and mesenteric lymph nodes collected from 404 bullocks, aged between 5 and 10years, were examined for JD lesions and detection of Mycobacterium avium subsp. paratuberculosis (Map). A total of 8.7% bullocks exhibited JD lesions, which were classified into multibacillary-diffuse granulomatous (n=2), paucibacillary-focal granulomatous (n=18) and paucibacillary-diffuse lymphocytic (n=15) lesions. The tissue cytokine gene expression profiles in all three forms of lesions corroborated with different immuno-pathological processes of JD in bullocks. The molecular typing and gene sequencing identified Map isolates from bullocks as bison type. •Pathological lesions of JD in bullocks were characterized.•Bacterial culture and PCR confirmed the prevalence of JD in bullocks.•Tissue cytokine gene expression was studied in bullocks positive for JD.•Molecular typing identified Map strains of bullocks as bison (B) type.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2016.03.009