Down-regulation of adenylate kinase 5 in temporal lobe epilepsy patients and rat model

Abstract Adenylate kinase 5 (AK5) is one member of the AK family and plays a critical role in maintaining cellular homeostasis. Different from the other AKs, AK5 is almost exclusively expressed in the brain. However, its exact biological functions remain unclear. The aim of the present study is to e...

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Bibliographic Details
Published in:Journal of the neurological sciences 2016-07, Vol.366, p.20-26
Main Authors: Lai, Yujie, Hu, Xiaotong, Chen, Guojun, Wang, Xuefeng, Zhu, Binglin
Format: Article
Language:English
Subjects:
Adenylate Kinase - metabolism
Adenylate kinase 5 (AK5)
Adolescent
Adult
Animals
Brain Injuries, Traumatic - enzymology
Brain Injuries, Traumatic - surgery
Carrier Proteins - metabolism
Child
Copine VI (CPNE6)
Disease Models, Animal
Down-Regulation
Drug Resistant Epilepsy - enzymology
Drug Resistant Epilepsy - surgery
Epilepsy, Temporal Lobe - enzymology
Epilepsy, Temporal Lobe - surgery
Female
Humans
Male
Microtubule-Associated Proteins - metabolism
Middle Aged
Nerve Tissue Proteins - metabolism
Neurology
Neurons - enzymology
Patients
Pilocarpine
Rat model
Rats, Sprague-Dawley
Refractory epilepsy
Young Adult
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Summary:Abstract Adenylate kinase 5 (AK5) is one member of the AK family and plays a critical role in maintaining cellular homeostasis. Different from the other AKs, AK5 is almost exclusively expressed in the brain. However, its exact biological functions remain unclear. The aim of the present study is to explore the expression pattern of AK5 in patients with refractory epilepsy and in a chronic pilocarpine-induced epileptic rat model. Using Western blot, immunofluorescence and immunoprecipitation analysis, we found that AK5 protein was mainly expressed in neurons, demonstrated by colocalization with the dendritic marker, MAP2, which were similar to the corresponding controls. However, the expression of AK5 decreased remarkably in epileptic patients and experimental rats. Furthermore, immunoprecipitation analysis showed that the interaction of AK5 with copine VI (CPNE6, a brain specific protein) increased in epileptic patients and rat models. Our results are the first to indicate that the expression of AK5 in epileptic brain tissue may play important roles in epilepsy, especially refractory epilepsy.
ISSN:0022-510X
1878-5883
DOI:10.1016/j.jns.2016.04.037