Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis

ABSTRACT Metastasis‐associated lung adenocarcinoma transcript 1 (Malat1) is an example of a functional long noncoding RNA involved in many biologic processes. However, the mechanisms for Malat1 in myogenesis are unclear. Serum response factor (SRF) is a pivotal transcription factor for muscle prolif...

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Bibliographic Details
Published in:The FASEB journal 2015-07, Vol.29 (7), p.3054-3064
Main Authors: Han, Xiaorui, Yang, Feng, Cao, Huiqing, Liang, Zicai
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Binding Sites - genetics
Binding, Competitive
Cell Differentiation
Cell Line
Gene Silencing
HEK293 Cells
Humans
long noncoding RNA
Mice
microRNA
MicroRNAs - genetics
MicroRNAs - metabolism
Models, Biological
Muscle Development - genetics
Muscle Development - physiology
Mutation
Myoblasts, Skeletal - cytology
Myoblasts, Skeletal - metabolism
RNA, Long Noncoding - antagonists & inhibitors
RNA, Long Noncoding - genetics
RNA, Long Noncoding - metabolism
Serum Response Factor - genetics
Serum Response Factor - metabolism
sponge
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Summary:ABSTRACT Metastasis‐associated lung adenocarcinoma transcript 1 (Malat1) is an example of a functional long noncoding RNA involved in many biologic processes. However, the mechanisms for Malat1 in myogenesis are unclear. Serum response factor (SRF) is a pivotal transcription factor for muscle proliferation and differentiation and is reported to be a target gene for muscle‐specific microRNA‐133 (miR‐133). In this study, we initially found that silencing Malat1 in the mouse myoblast C2C12 cell line inhibited myocyte differentiation and decreased Srf at both the RNA and protein levels. Srf silencing decreased Malat1 expression as well. Further study revealed that Malat1 contained an miR‐133 functional target site, and the interplay between Malat1 and Srf was miR‐133 dependent. We demonstrated that Malat1 modulates Srf through miR‐133 as a competing endogenous RNA and established a novel connection among Malat1, miR‐133, and Srf in myoblast differentiation.— Han, X., Yang, F., Cao, H., Liang. Z. Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis. FASEB J. 29, 3054‐3064 (2015). www.fasebj.org
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.14-259952