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Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis
ABSTRACT Metastasis‐associated lung adenocarcinoma transcript 1 (Malat1) is an example of a functional long noncoding RNA involved in many biologic processes. However, the mechanisms for Malat1 in myogenesis are unclear. Serum response factor (SRF) is a pivotal transcription factor for muscle prolif...
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| Published in: | The FASEB journal 2015-07, Vol.29 (7), p.3054-3064 |
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| container_title | The FASEB journal |
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| creator | Han, Xiaorui Yang, Feng Cao, Huiqing Liang, Zicai |
| description | ABSTRACT
Metastasis‐associated lung adenocarcinoma transcript 1 (Malat1) is an example of a functional long noncoding RNA involved in many biologic processes. However, the mechanisms for Malat1 in myogenesis are unclear. Serum response factor (SRF) is a pivotal transcription factor for muscle proliferation and differentiation and is reported to be a target gene for muscle‐specific microRNA‐133 (miR‐133). In this study, we initially found that silencing Malat1 in the mouse myoblast C2C12 cell line inhibited myocyte differentiation and decreased Srf at both the RNA and protein levels. Srf silencing decreased Malat1 expression as well. Further study revealed that Malat1 contained an miR‐133 functional target site, and the interplay between Malat1 and Srf was miR‐133 dependent. We demonstrated that Malat1 modulates Srf through miR‐133 as a competing endogenous RNA and established a novel connection among Malat1, miR‐133, and Srf in myoblast differentiation.— Han, X., Yang, F., Cao, H., Liang. Z. Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis. FASEB J. 29, 3054‐3064 (2015). www.fasebj.org |
| doi_str_mv | 10.1096/fj.14-259952 |
| format | article |
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Metastasis‐associated lung adenocarcinoma transcript 1 (Malat1) is an example of a functional long noncoding RNA involved in many biologic processes. However, the mechanisms for Malat1 in myogenesis are unclear. Serum response factor (SRF) is a pivotal transcription factor for muscle proliferation and differentiation and is reported to be a target gene for muscle‐specific microRNA‐133 (miR‐133). In this study, we initially found that silencing Malat1 in the mouse myoblast C2C12 cell line inhibited myocyte differentiation and decreased Srf at both the RNA and protein levels. Srf silencing decreased Malat1 expression as well. Further study revealed that Malat1 contained an miR‐133 functional target site, and the interplay between Malat1 and Srf was miR‐133 dependent. We demonstrated that Malat1 modulates Srf through miR‐133 as a competing endogenous RNA and established a novel connection among Malat1, miR‐133, and Srf in myoblast differentiation.— Han, X., Yang, F., Cao, H., Liang. Z. Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis. FASEB J. 29, 3054‐3064 (2015). www.fasebj.org</description><identifier>ISSN: 0892-6638</identifier><identifier>EISSN: 1530-6860</identifier><identifier>DOI: 10.1096/fj.14-259952</identifier><identifier>PMID: 25868726</identifier><language>eng</language><publisher>United States: Federation of American Societies for Experimental Biology</publisher><subject>Animals ; Base Sequence ; Binding Sites - genetics ; Binding, Competitive ; Cell Differentiation ; Cell Line ; Gene Silencing ; HEK293 Cells ; Humans ; long noncoding RNA ; Mice ; microRNA ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Models, Biological ; Muscle Development - genetics ; Muscle Development - physiology ; Mutation ; Myoblasts, Skeletal - cytology ; Myoblasts, Skeletal - metabolism ; RNA, Long Noncoding - antagonists & inhibitors ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; Serum Response Factor - genetics ; Serum Response Factor - metabolism ; sponge</subject><ispartof>The FASEB journal, 2015-07, Vol.29 (7), p.3054-3064</ispartof><rights>FASEB</rights><rights>FASEB.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4112-3bc0189d33abc081f7a88d17cd0b4b888e2e6265e7d6965aeb3f8d070951c4753</citedby><cites>FETCH-LOGICAL-c4112-3bc0189d33abc081f7a88d17cd0b4b888e2e6265e7d6965aeb3f8d070951c4753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25868726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Han, Xiaorui</creatorcontrib><creatorcontrib>Yang, Feng</creatorcontrib><creatorcontrib>Cao, Huiqing</creatorcontrib><creatorcontrib>Liang, Zicai</creatorcontrib><title>Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis</title><title>The FASEB journal</title><addtitle>FASEB J</addtitle><description>ABSTRACT
Metastasis‐associated lung adenocarcinoma transcript 1 (Malat1) is an example of a functional long noncoding RNA involved in many biologic processes. However, the mechanisms for Malat1 in myogenesis are unclear. Serum response factor (SRF) is a pivotal transcription factor for muscle proliferation and differentiation and is reported to be a target gene for muscle‐specific microRNA‐133 (miR‐133). In this study, we initially found that silencing Malat1 in the mouse myoblast C2C12 cell line inhibited myocyte differentiation and decreased Srf at both the RNA and protein levels. Srf silencing decreased Malat1 expression as well. Further study revealed that Malat1 contained an miR‐133 functional target site, and the interplay between Malat1 and Srf was miR‐133 dependent. We demonstrated that Malat1 modulates Srf through miR‐133 as a competing endogenous RNA and established a novel connection among Malat1, miR‐133, and Srf in myoblast differentiation.— Han, X., Yang, F., Cao, H., Liang. Z. Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis. FASEB J. 29, 3054‐3064 (2015). www.fasebj.org</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>Binding, Competitive</subject><subject>Cell Differentiation</subject><subject>Cell Line</subject><subject>Gene Silencing</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>long noncoding RNA</subject><subject>Mice</subject><subject>microRNA</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Models, Biological</subject><subject>Muscle Development - genetics</subject><subject>Muscle Development - physiology</subject><subject>Mutation</subject><subject>Myoblasts, Skeletal - cytology</subject><subject>Myoblasts, Skeletal - metabolism</subject><subject>RNA, Long Noncoding - antagonists & inhibitors</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>Serum Response Factor - genetics</subject><subject>Serum Response Factor - metabolism</subject><subject>sponge</subject><issn>0892-6638</issn><issn>1530-6860</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1EAMhkcIRLcfN85ojhxIsWcyE-dYKraACpX6cR5NEmebVT6WmUTV3vgJ_Y38ErLahSOc_Np69MiyhXiDcI6Q2w_1-hzTRJk8N-qFWKDRkFiy8FIsgHKVWKvpSBzHuAYABLSvxZEyZClTdiGqb771I8rAq2kOHGXkMHVzHzdDH1nWvhyHIMfHMEyrR9k1t79-PqPW0kfpZTl0Gx6bfiW5r4YV98MU5e33C9n0stvuBhybeCpe1b6NfHaoJ-Jh-en-8nNyfXP15fLiOilTRJXoogSkvNLaz4mwzjxRhVlZQZEWRMSKrbKGs8rm1ngudE0VZJAbLNPM6BPxbu_dhOHHxHF0XRNLblvf87yYQwKyqQKb_x-dGSQDdmd9v0fLMMQYuHab0HQ-bB2C273A1WuHqdu_YMbfHsxT0XH1F_5z8xmgPfDUtLz9p8wt7z6q5VdMD-7fDv-SOw</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Han, Xiaorui</creator><creator>Yang, Feng</creator><creator>Cao, Huiqing</creator><creator>Liang, Zicai</creator><general>Federation of American Societies for Experimental Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7TM</scope><scope>H94</scope></search><sort><creationdate>201507</creationdate><title>Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis</title><author>Han, Xiaorui ; Yang, Feng ; Cao, Huiqing ; Liang, Zicai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4112-3bc0189d33abc081f7a88d17cd0b4b888e2e6265e7d6965aeb3f8d070951c4753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Binding Sites - genetics</topic><topic>Binding, Competitive</topic><topic>Cell Differentiation</topic><topic>Cell Line</topic><topic>Gene Silencing</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>long noncoding RNA</topic><topic>Mice</topic><topic>microRNA</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Models, Biological</topic><topic>Muscle Development - genetics</topic><topic>Muscle Development - physiology</topic><topic>Mutation</topic><topic>Myoblasts, Skeletal - cytology</topic><topic>Myoblasts, Skeletal - metabolism</topic><topic>RNA, Long Noncoding - antagonists & inhibitors</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>Serum Response Factor - genetics</topic><topic>Serum Response Factor - metabolism</topic><topic>sponge</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Han, Xiaorui</creatorcontrib><creatorcontrib>Yang, Feng</creatorcontrib><creatorcontrib>Cao, Huiqing</creatorcontrib><creatorcontrib>Liang, Zicai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The FASEB journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Han, Xiaorui</au><au>Yang, Feng</au><au>Cao, Huiqing</au><au>Liang, Zicai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis</atitle><jtitle>The FASEB journal</jtitle><addtitle>FASEB J</addtitle><date>2015-07</date><risdate>2015</risdate><volume>29</volume><issue>7</issue><spage>3054</spage><epage>3064</epage><pages>3054-3064</pages><issn>0892-6638</issn><eissn>1530-6860</eissn><abstract>ABSTRACT
Metastasis‐associated lung adenocarcinoma transcript 1 (Malat1) is an example of a functional long noncoding RNA involved in many biologic processes. However, the mechanisms for Malat1 in myogenesis are unclear. Serum response factor (SRF) is a pivotal transcription factor for muscle proliferation and differentiation and is reported to be a target gene for muscle‐specific microRNA‐133 (miR‐133). In this study, we initially found that silencing Malat1 in the mouse myoblast C2C12 cell line inhibited myocyte differentiation and decreased Srf at both the RNA and protein levels. Srf silencing decreased Malat1 expression as well. Further study revealed that Malat1 contained an miR‐133 functional target site, and the interplay between Malat1 and Srf was miR‐133 dependent. We demonstrated that Malat1 modulates Srf through miR‐133 as a competing endogenous RNA and established a novel connection among Malat1, miR‐133, and Srf in myoblast differentiation.— Han, X., Yang, F., Cao, H., Liang. Z. Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis. FASEB J. 29, 3054‐3064 (2015). www.fasebj.org</abstract><cop>United States</cop><pub>Federation of American Societies for Experimental Biology</pub><pmid>25868726</pmid><doi>10.1096/fj.14-259952</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Base Sequence Binding Sites - genetics Binding, Competitive Cell Differentiation Cell Line Gene Silencing HEK293 Cells Humans long noncoding RNA Mice microRNA MicroRNAs - genetics MicroRNAs - metabolism Models, Biological Muscle Development - genetics Muscle Development - physiology Mutation Myoblasts, Skeletal - cytology Myoblasts, Skeletal - metabolism RNA, Long Noncoding - antagonists & inhibitors RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Serum Response Factor - genetics Serum Response Factor - metabolism sponge |
| title | Malat1 regulates serum response factor through miR‐133 as a competing endogenous RNA in myogenesis |
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