Sensitivity and specificity of the empirical lymphocyte genome sensitivity (LGS) assay: implications for improving cancer diagnostics

Lymphocyte responses from 208 individuals: 20 with melanoma, 34 with colon cancer, and 4 with lung cancer (58), 18 with suspected melanoma, 28 with polyposis, and 10 with COPD (56), and 94 healthy volunteers were examined. The natural logarithm of the Olive tail moment (OTM) was plotted for exposure...

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Bibliographic Details
Published in:The FASEB journal 2014-10, Vol.28 (10), p.4563-4570
Main Authors: Anderson, Diana, Najafzadeh, Mojgan, Gopalan, Rajendran, Ghaderi, Nader, Scally, Andrew J., Britland, Stephen T., Jacobs, Badie K., Reynolds, P. Dominic, Davies, Justin, Wright, Andrew L., Al‐Ghazal, Shariff, Sharpe, David, Denyer, Morgan C.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
blood test
Case-Control Studies
Data Interpretation, Statistical
Early Detection of Cancer - methods
Female
Genome, Human
Humans
Lymphocytes - radiation effects
Male
Middle Aged
Neoplasms - diagnosis
Olea
Radiation Tolerance
Sensitivity and Specificity
susceptibility
Ultraviolet Rays
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Summary:Lymphocyte responses from 208 individuals: 20 with melanoma, 34 with colon cancer, and 4 with lung cancer (58), 18 with suspected melanoma, 28 with polyposis, and 10 with COPD (56), and 94 healthy volunteers were examined. The natural logarithm of the Olive tail moment (OTM) was plotted for exposure to UVA through 5 different agar depths (100 cell measurements/depth) and analyzed using a repeated measures regression model. Responses of patients with cancer plateaued after treatment with different UVA intensities, but returned toward control values for healthy volunteers. For precancerous conditions and suspected cancers, intermediate responses occurred. ROC analysis of mean log OTMs, for cancers plus precancerous/suspect conditions vs. controls, cancer vs. precancerous/suspect conditions plus controls, and cancer vs. controls, gave areas under the curve of 0.87, 0.89, and 0.93, respectively (P
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.14-254748