Advanced lymphoblastic clones detection in T-cell leukemia

T cell acute lymphoblastic leukemia (T-ALL) is an aggressive malignant neoplasm of the lymphocyte precursors that suffered malignant transformation arresting the lymphoid cell differentiation. Clinical studies revealed monoor, more rarely, oligoclonal nature of the disease. A precise identification...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2016-03, Vol.467 (1), p.85-88
Main Authors: Minervina, A. A., Komkov, A. Y., Mamedov, I. Z., Lebedev, Y. B.
Format: Article
Language:English
Subjects:
Biochemistry
Biological and Medical Physics
Biomarkers
Biomedical and Life Sciences
Biophysics
Biophysics and Molecular Biology
Bone Marrow - metabolism
Cellular biology
Cloning
DNA Primers
Electrophoresis
Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
Genetic Loci
Humans
Jurkat Cells
Leukemia
Leukemia, T-Cell - diagnosis
Leukemia, T-Cell - genetics
Leukemia, T-Cell - metabolism
Life Sciences
Neoplasm, Residual
Polymerase Chain Reaction - methods
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
V(D)J Recombination
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Summary:T cell acute lymphoblastic leukemia (T-ALL) is an aggressive malignant neoplasm of the lymphocyte precursors that suffered malignant transformation arresting the lymphoid cell differentiation. Clinical studies revealed monoor, more rarely, oligoclonal nature of the disease. A precise identification of malignant clone markers is both the crucial stage of early diagnostics and the essential prognostic factor for therapeutic treatment. Here we present an improved system for unbiased detection of lymphoblastic clones in bone marrow aspirates of T-ALL patients. The system based on multiplex PCR of rearranged T-cell receptor locus (TRB) and straightforward sequencing of the resulted PCR fragments. Testing of the system on genomic DNA from Jurkat cell line and four clinical bone marrow aspirates revealed a set of unique TRB rearrangements that precisely characterize each of tested samples. Therefore, the outcome of the system produces highly informative molecular genetic markers for further monitoring of minimal residual disease in T-ALL patients.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672916020022