Native plant growth promoting bacteria Bacillus thuringiensis and mixed or individual mycorrhizal species improved drought tolerance and oxidative metabolism in Lavandula dentata plants

• Published in: Journal of plant physiology 2016-03, Vol.192, p.1-12
• Main Authors: Armada, E., Probanza, A., Roldán, A., Azcón, R.
• Format: Article
• Language: English
• Subjects: Antioxidants - metabolism; Autochthonous bacterial strain; Autochthonous mycorrhizal fungi; Bacillus thuringiensis; Bacillus thuringiensis - physiology; Biomass; Dehydration; Droughts; Enzymatic antioxidants; Lavandula; Lavandula - growth & development; Lavandula - metabolism; Lavandula - microbiology; Lavandula - ultrastructure; Mycorrhizae - growth & development; Mycorrhizae - physiology; Oxidative damage; Oxidative Stress; Plant Roots - growth & development; Plant Roots - metabolism; Plant Roots - microbiology; Plant Roots - ultrastructure; Plant Shoots - growth & development; Plant Shoots - metabolism; Plant Shoots - microbiology; Plant Shoots - ultrastructure; Symbiosis; Water stress
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2015.11.007

This study evaluates the responses of Lavandula dentata under drought conditions to the inoculation with single autochthonous arbuscular mycorrhizal (AM) fungus (five fungal strains) or with their mixture and the effects of these inocula with a native Bacillus thuringiensis (endophytic bacteria). These microorganisms were drought tolerant and in general, increased plant growth and nutrition. Particularly, the AM fungal mixture and B. thuringiensis maximized plant biomass and compensated drought stress as values of antioxidant activities [superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase APX)] shown. The AMF-bacteria interactions highly reduced the plant oxidative damage of lipids [malondialdehyde (MDA)] and increased the mycorrhizal development (mainly arbuscular formation representative of symbiotic functionality). These microbial interactions explain the highest potential of dually inoculated plants to tolerate drought stress. B. thuringiensis “in vitro” under osmotic stress does not reduce its PGPB (plant growth promoting bacteria) abilities as indole acetic acid (IAA) and ACC deaminase production and phosphate solubilization indicating its capacity to improve plant growth under stress conditions. Each one of the autochthonous fungal strains maintained their particular interaction with B. thuringiensis reflecting the diversity, intrinsic abilities and inherent compatibility of these microorganisms. In general, autochthonous AM fungal species and particularly their mixture with B. thuringiensis demonstrated their potential for protecting plants against drought and helping plants to thrive in semiarid ecosystems.