α-Ketol linolenic acid (KODA) application affects endogenous abscisic acid, jasmonic acid and aromatic volatiles in grapes infected by a pathogen (Glomerella cingulata)

• Published in: Journal of plant physiology 2016-03, Vol.192, p.90-97
• Main Authors: Wang, Shanshan, Saito, Takanori, Ohkawa, Katsuya, Ohara, Hitoshi, Shishido, Masahiro, Ikeura, Hiromi, Takagi, Kazuteru, Ogawa, Shigeyuki, Yokoyama, Mineyuki, Kondo, Satoru
• Format: Article
• Language: English
• Subjects: Abscisic Acid - metabolism; Aldehydes - metabolism; alpha-Linolenic Acid - metabolism; Antioxidant activity; Antioxidants - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; C6-aldehyde; Cyclopentanes - metabolism; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Dioxygenases - genetics; Dioxygenases - metabolism; Fruit - genetics; Fruit - immunology; Fruit - microbiology; Fruit - physiology; Glomerella cingulata; Intramolecular Oxidoreductases - genetics; Intramolecular Oxidoreductases - metabolism; Ketol linolenic acid; Lipoxygenase - genetics; Lipoxygenase - metabolism; Oxylipins - metabolism; Pathogen; Phyllachorales - physiology; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Growth Regulators - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Signal Transduction; Vitaceae; Vitis - genetics; Vitis - immunology; Vitis - microbiology; Vitis - physiology; Vitis labrusca; Vitis labrusca × Vitis vinifera; Vitis vinifera
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2016.01.009

Effects of α-ketol linolenic acid (KODA) application on endogenous abscisic acid (ABA), jasmonic acid (JA), and aromatic volatiles were investigated in ‘Kyoho’ grapes (Vitis labrusca×Vitis vinifera) infected by a pathogen (Glomerella cingulata). The expressions of 9-cis-epoxycarotenoid dioxygenase (VvNCED1), ABA 8′-hydroxylase (VvCYP707A1), lipoxygenase (VvLOX), and allene oxide synthase (VvAOS) were also examined. The grape berries were dipped in 0.1mM KODA solution before inoculation with the pathogen and stored at 25°C for 12 days. The development of infection was significantly suppressed upon KODA treatment. Endogenous ABA, JA and phaseic acid (PA) were induced in inoculated berries. KODA application before inoculation increased endogenous ABA, PA and JA through the activation of VvNCED1, VvCYP707A1 and VvAOS genes, respectively. In addition, terpenes, methyl salicylate (Me-SA) and C6-aldehydes such as (E)-2-hexenal and cis-3-hexenal associated with fungal resistance also increased in KODA-treated berries during storage. These results suggest that the synergistic effect of JA, ABA, and some aromatic volatiles induced by KODA application may provide resistance to pathogen infection in grape berries.