Bisphenol A exposure and healing effects of Adiantum capillus-veneris L. plant extract (APE) in bisphenol A-induced reproductive toxicity in albino rats

• Published in: Environmental science and pollution research international 2016-06, Vol.23 (12), p.11645-11657
• Main Authors: Yousaf, Balal, Amina, Liu, Guijian, Wang, Ruwei, Qadir, Abdul, Ali, Muhammad Ubaid, Kanwal, Qudsia, Munir, Bushra, Asmatullah, Abbas, Zaigham
• Format: Article
• Language: English
• Subjects: Adiantum; Adiantum capillus-veneris; Animals; Antioxidants - pharmacology; Antioxidants - therapeutic use; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Basement membranes; Benzhydryl Compounds - toxicity; Bisphenol A; Body weight; Developmental biology; Diabetes; Distilled water; DNA methylation; Earth and Environmental Science; Ecotoxicology; Endocrine Disruptors - toxicity; Environment; Environmental Chemistry; Environmental Health; Environmental science; Estrogens, Non-Steroidal - toxicity; Food; Leydig cells; Male; Male reproductive system; Medical research; Olea; Olive oil; Oral administration; Organ Size - drug effects; Phenols - toxicity; Plant extracts; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Rats; Reproduction - drug effects; Reproductive system; Research Article; Rodents; Spermatocytes; Spermatogenesis; Studies; Testes; Testis - drug effects; Testis - pathology; Testosterone; Testosterone - blood; Toxicity; Toxicology; Tubules; Waste Water Technology; Water Management; Water Pollution Control; Xenoestrogens; Zoology
• ISSN: 0944-1344; 1614-7499
• DOI: 10.1007/s11356-016-6330-0

The current study presents the bisphenol A exposure and the ameliorative effects of Adiantum capillus-veneris on testicular toxicity induced by bisphenol A. Adult male albino rats were divided into five groups of five animals each: A (control), B (vehicle control), C (toxic), D (protective), and E (ameliorative) were served distilled water, olive oil, bisphenol A (BPA) at 100 mg/kg body weight, A. capillus-veneris plant extract at 25 mg/kg body weight, and BPA + A. capillus-veneris , respectively. All of the doses were administered orally for 15 days, and the rats were then sacrificed. Blood samples for the testosterone assay and both testes were collected for histological examination. The body weight, paired testes weight, relative tissue weight index, Johnsen scoring of tubules, and level of serum testosterone decreased in BPA-treated rats. Similarly, histological examination of the testes in BPA-treated animals revealed a lower number of Leydig cells, an irregular basement membrane, sloughing of germinal layers, vacuolization, a lower number of spermatocytes, and debris in the lumen. However, co-administration of A. capillus-veneris with BPA increased the total antioxidative capacity (330.82 ± 22.46 μmol/mg protein) of the testes and restored the serum testosterone level (1.70 ng/ml); histological features showed restoration in the stages of spermatogenesis. Conclusively, A. capillus-veneris plant extract overcomes the estrogenic effects of BPA on the reproductive system of rats and protects rats’ testes against BPA-induced injury/damage via an antioxidative mechanism that appears to be conciliated.