The Effect of Intravenous Immunoglobulin (IVIG) on In vitro Activation of Circulating Neutrophils from CBA and C57BL/6 Mice with Fibrosarcoma S37

• Published in: Immunome research 2013, Vol.9 (1), p.1-1
• Main Authors: Basyreva, Liliya Yu, Brodsky, Ilya B, Gusev, Alexandr A, Zhapparova, Olga N, Mikhalchik, Elena V, Gusev, Sergey A, Matishov, Dmitriy G, Blank, Miri, Shoenfeld, Yehudta
• Format: Article
• Language: English
• ISSN: 1745-7580; 1745-7580
• DOI: 10.4172/1745-7580.1000065

The application of IVIG for cancer treatment is associated with the problem of variable patient's response to IVIG, which might result from the peculiarities of neutrophil's activation. Individual variations in the response to IVIG treatment can be elucidated by the comparison of CBA and C57BL/6 mouse strains. These two strains are considered as contrasting ones regarding function of their immune system. Here we studied zymosan-induced activation of circulating neutrophils from CBA and C57BL/6 mice developing fibrosarcoma. The circulating neutrophils of CBA and C57BL/6 mice were studied at early stages of tumor growth. The WBC total and differential counting revealed the increase of neutrophil content in blood up to the day 7 after fibrosarcoma cells S37 inoculation. The neutrophils' activity was measured as opsonized-stimulated (OZ) chemiluminiscent (CL) response of whole blood samples at various stages of tumor growth. As for normal neutrophils this effect was observed only in the presence of extracellular calcium. At day 7 after inoculation IVIG inhibited in vitro CL responses of blood samples stimulated with OZ.