Functional role of arginine during the peri‐implantation period of pregnancy. I. Consequences of loss of function of arginine transporter SLC7A1 mRNA in ovine conceptus trophectoderm

ABSTRACT Arginine, the common substrate for production of nitric oxide (NO) and polyamines in mammals, increases in the uterine lumen during the peri‐implantation period of pregnancy. However, functional roles of arginine within the uterine lumen for conceptus (embryo and extraembryonic membranes) d...

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Bibliographic Details
Published in:The FASEB journal 2014-07, Vol.28 (7), p.2852-2863
Main Authors: Wang, Xiaoqiu, Frank, James W., Little, Danielle R., Dunlap, Kathrin A., Satterfield, M. Carey, Burghardt, Robert C., Hansen, Thomas R., Wu, Guoyao, Bazer, Fuller W.
Format: Article
Language:English
Subjects:
Amino Acids - genetics
Amino Acids - metabolism
Animals
Arginine - genetics
Arginine - metabolism
Cationic Amino Acid Transporter 1 - genetics
Cationic Amino Acid Transporter 1 - metabolism
development
Embryo Implantation - genetics
Embryo Implantation - physiology
Endometrium - metabolism
Endometrium - physiology
Female
Gene Expression Regulation, Developmental - genetics
morpholino
Nitric Oxide Synthase Type III - genetics
Nitric Oxide Synthase Type III - metabolism
Ornithine Decarboxylase - genetics
Ornithine Decarboxylase - metabolism
Polyamines - metabolism
Pregnancy
RNA, Messenger - genetics
sheep
Sheep - genetics
Sheep - metabolism
Uterus - metabolism
Uterus - physiology
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Summary:ABSTRACT Arginine, the common substrate for production of nitric oxide (NO) and polyamines in mammals, increases in the uterine lumen during the peri‐implantation period of pregnancy. However, functional roles of arginine within the uterine lumen for conceptus (embryo and extraembryonic membranes) development have not been elucidated in vivo. To assess roles of arginine in reproductive tissue for survival and development of the conceptus, we conducted an in vivo morpholino antisense oligonucleotide (MAO)‐mediated knockdown of SLC7A1 mRNA, the arginine transporter in ovine conceptus trophectoderm (Tr). Translational knockdown of SLC7A1 mRNA resulted in retarded conceptus development and abnormal function compared to MAO control. Use of MAO‐SLC7A1 knockdown in conceptuses decreased arginine transport (73%, P
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.13-248757