Detection of West Nile virus and tick-borne encephalitis virus in birds in Slovakia, using a universal primer set

West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic p...

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Bibliographic Details
Published in:Archives of virology 2016-06, Vol.161 (6), p.1679-1683
Main Authors: Csank, Tomáš, Bhide, Katarína, Bencúrová, Elena, Dolinská, Saskia, Drzewnioková, Petra, Major, Peter, Korytár, Ľuboš, Bocková, Eva, Bhide, Mangesh, Pistl, Juraj
Format: Article
Language:English
Subjects:
Animals
Antibodies
Arachnids
Biomedical and Life Sciences
Biomedicine
Bird Diseases - virology
Bird migration
Birds - virology
Brief Report
Disease Vectors
DNA Primers - genetics
Encephalitis
Encephalitis Viruses, Tick-Borne - genetics
Encephalitis Viruses, Tick-Borne - isolation & purification
Encephalitis, Tick-Borne - transmission
Flavivirus
Horses
Humans
Infectious Diseases
Internet resources
Medical Microbiology
Mosquitoes
Pharmacy
Phylogenetics
Phylogeny
RNA, Viral - genetics
RNA, Viral - isolation & purification
Slovakia
Tick-borne encephalitis virus
Veterinary medicine
Virology
Viruses
West Nile Fever - transmission
West Nile Fever - veterinary
West Nile virus
West Nile virus - classification
West Nile virus - genetics
West Nile virus - isolation & purification
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Summary:West Nile virus (WNV) is a mosquito-borne neurotropic pathogen that presents a major public health concern. Information on WNV prevalence and circulation in Slovakia is insufficient. Oral and cloacal swabs and bird brain samples were tested for flavivirus RNA by RT-PCR using newly designed generic primers. The species designation was confirmed by sequencing. WNV was detected in swab and brain samples, whereas one brain sample was positive for tick-borne encephalitis virus (TBEV). The WNV sequences clustered with lineages 1 and 2. These results confirm the circulation of WNV in birds in Slovakia and emphasize the risk of infection of humans and horses.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-016-2828-5